Mag-Bind® Endo-free Plasmid Midi Kit

on the Dynamic Devices’ Lynx

Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 50 mL culture volume.

Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
High-yielding – Plasmid DNA yield up to 200 μg
Rapid – 24 samples can be processed in ~ 70 minutes starting from a bacterial pellet without manual intervention
Versatile – Process up to 50 mL bacterial culture with different plasmid types and culture medias
Transfection-quality – Endotoxin free plasmid DNA with endotoxins levels < 0.1 EU/µg
Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8

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Mag-Bind® Endo-free Plasmid Midi Kit

Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 50 mL culture volume.

Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
High-yielding – Plasmid DNA yield up to 200 μg
Rapid – 24 samples can be processed in ~ 70 minutes starting from a bacterial pellet without manual intervention
Versatile – Process up to 50 mL bacterial culture with different plasmid types and culture medias
Transfection-quality – Endotoxin free plasmid DNA with endotoxins levels < 0.1 EU/µg
Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8

Automated Protocol Data

Comparable Plasmid Integrity Regardless of Extraction Method

Figure 1. pGEM Plasmid DNA was extracted from 50 mL bacterial pellet manually using the Mag-Bind® Endo-free Plasmid Midi Kit, according to manufacturer’s instructions, as well as using the Kit automated on Dynamic Devices’ Lynx platform. TapeStation analysis shows comparable integrity between the two methods.

Consistent Plasmid DNA Yields with Excellent Purity Ratios

Figure 2. pGEM Plasmid DNA was extracted from 50 mL bacterial pellet manually using the Mag-Bind® Endo-free Plasmid Midi Kit, according to manufacturer’s instructions, as well as using the Kit automated on Dynamic Devices’ Lynx platform. Average yields were 1.5X higher for automated extraction vs. manual. A260/A280 and A260/A230 ratios were comparable between the two methods.

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

Scripts available for all Mag-Bind® Kits for most open liquid handlers and common magnetic processors including the MagBinder® Fit²⁴. Visit Automated Solutions for more information.