Mag-Bind® NGS Normalization Kit

The Mag-Bind® NGS Normalization Kit is designed for normalizing libraries for use in Next Generation Sequencing workflows. The Kit follows magnetic bead-based methodology to facilitate normalization of libraries or PCR amplicons starting with at least 200 ng input and resulting in ~100 ng normalized output. The Kit eliminates manual library quantification and dilution, reducing pipetting steps, time, and tip costs.

The Mag-Bind® NGS Normalization Kit follows an easy and fast workflow and is compatible with most open -ended automation platforms. The protocol follows a simple bind -wash-elute routine. Briefly, the libraries at different concentrations are bound to
Mag-Bind® Particles DDB in the presence of a bindi ng buffer. The magnetic beads have a limited binding capacity allowing only a predetermined amount of DNA to be captured and eluted to a fixed concentration. The output concentration can be adjusted by adjusting the elution volume used. Once normalized, the different libraries can be pooled at equal volumes so that they are evenly represented in downstream sequencing workflows.