Mag-Bind® Total RNA Xpress Kit

The Mag-Bind® Total RNA Xpress Kit allows for rapid and reliable isolation of high-quality total RNA including small RNAs from a wide variety of tissue and cultured cells. Total RNA can be purified from up to 25 mg tissue or 1 x 10 6 cultured cells. The Kit
follows most magnetic bead-based technology and can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors.

If using the Mag -Bind® Total RNA Xpress Kit for the first time, please read this manual in its entirety to become familiar with the procedures. Samples are first lysed in OTRK Lysis Buffer supplemented with DTT. The lysate is cleared using centrifugation and
total nucleic acids are then bound to Mag -Bind® Particles RQ in the presence of isopropanol. Genomic DNA bound to the beads is eliminated through a DNase I digestion step while preserving the integrity of the RNA bound to the beads. The beads are subjected to two quick alcohol washes to remove contaminants. High-quality RNA is then eluted in Nuclease-free Water and is ready for use in a wide range of downstream applications such as RT-PCR, RT-qPCR, microarray analysis, next-generation RNA sequencing (RNA-Seq), etc.