Reliable DNA Template Preparation Improves Cell-Free Protein Production from E. coli Plasmid Samples
Producing consistent, high-quality proteins in cell-free expression (CFE) systems depends on more than just the reaction mix—it also hinges on how DNA templates are prepared. In a 2022 study published in Synthetic Biology, researchers from the U.S. National Institute of Standards and Technology (NIST) systematically examined how different DNA extraction workflows influence variability in cell-free […]
