$0.00 – $2,329.30
Sequential isolation of both DNA and RNA from the same FFPE sample using magnetic beads
All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.
Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.
Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.
Mag-Bind® FFPE DNA/RNA 96 Kit is designed for sequentially isolating DNA and RNA from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample. The protocol utilizes non-toxic mineral oil in combination with heat for efficient deparaffinization of the FFPE sample, eliminating the use of hazardous xylene.
The specially formulated buffers reverse cross-linking without the need for overnight digestion, resulting in high-yielding, high-quality nucleic acids. The isolation protocol allows for the extraction of DNA and RNA in separate eluates from the same sample for a comprehensive analysis of both nucleic acids. Purified DNA and RNA are suitable for a variety of downstream applications, including SNP analysis, sequencing, and genotyping.
Mag-Bind® system is fully automatable on Hamilton Microlab® STAR™, Tecan Freedom Evo®, Thermo Fisher Scientific KingFisher® Flex Purification System, and other open-ended workstations.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Applications | NGS, PCR, qPCR, real-time RT-PCR, microarray, microRNA analysis |
| Elution Volume | 50-200 µL |
| Starting Material | FFPE tissue |
| Starting Amount | < 3 FFPE sections of 10 μm thickness each |
| Throughput | Up to 96 |
| Processing Mode | Automated, Manual |
| Technology | Magnetic beads |
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| FDR Buffer | – – – |
| Proteinase K Solution | View Product |
| MB4 Buffer | – – – |
| Mag-Bind® Particles CH | – – – |
| RMP Buffer | – – – |
| Elution Buffer | View Product |
| DNase Digestion Buffer | – – – |
| Mag-Bind® DNase I | – – – |
| PHM Buffer | – – – |
| Nuclease-free Water | View Product |
Figure 1. Average DNA (A) and RNA (B) yields from FFPE tumor samples. Genomic DNA (A) and RNA (B) was sequentially isolated from the same 1 × 10 μm section of the FFPE tumor tissue sample (n=3) using Omega Bio-tek’s Mag-Bind® FFPE DNA/RNA 96 Kit and comparable kits from Company T and Company Q following manufacturer’s recommended protocols. Purified DNA and RNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system as well as Promega’s QuantiFluor® dsDNA and RNA system.
Figure 2. Purity of DNA (A) and RNA (B) isolated using different manufacturer’s kits was analyzed through spectrophotometry focusing on A260/A280 and A260/A230 ratios.
Figure 3. Purity of DNA (A) and RNA (B) isolated using different manufacturer’s kits was analyzed through spectrophotometry focusing on A260/A280 and A260/A230 ratios.
Table 1. Average DV200 value (percentage of fragments >200 nt) of RNA purified using different kits analyzed on Agilent’s TapeStation® 2200.
Table 3. ΔCq values of DNA extracted from FFPE and non-FFPE colorectal tumor tissue samples using kits from different manufacturers.
Table 3. Omega Bio-tek’s Mag-Bind FFPE DNA/RNA 96 Kit showed a higher percentage of aligned reads compared to Kits from Company T and Company Q across breast, lung, and colorectal tumor tissue types
Figure 4. Small Variant Calling Analysis on DNA from FFPE colorectal tumor tissue sample extracted from different kits compared to fresh frozen control.
