E-Z 96™ Cycle Pure Kit
- Rapid – Purification of up to 96 PCR samples in 30 minutes
- Efficient – Over 85% recovery of PCR fragments
- Convenient – No phenol/chloroform extractions
- Automation-friendly – E-Z 96™ DNA plates fit precisely into the VAC-03 vacuum manifold and most standard 96-well plate manifolds for convenient parallel sample processing
- High quality – DNA is suitable for downstream applications
Product has been discontinued. Mag-Bind® TotalPure NGS is the recommended alternative.
SKU#: D1043 (5 x 96 Preps)
The E-Z 96™ Cycle Pure Kit procedure allows for the parallel purification of up to 96 PCR samples from multiple amplifications. The E-Z 96™ Cycle Pure Kit utilizes multiwell technology for manual or fully automated high-throughput purification. The HiBind® DNA resin selectively adsorbs DNA molecules in an optimized binding buffer (Buffer CP). After a single wash step, pure PCR products are eluted in water or Elution Buffer provided and are ready to use in subsequent applications.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream application | Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| Elution volume | 80-100 µL |
| Starting material | PCR reaction |
| Starting amount | Up to 12 µg DNA |
| DNA recovered | >90% recovery, 70 bp to 20 kb |
| Processing mode | Manual, centrifugation/vacuum |
| Throughput | 96 samples per run |
| DNA binding technology | Silica 96-well plate |
| Binding capacity | 12 μg |
| Processing time | <15 minutes |
Figure 1. Analysis of PCR products before (c) and after (1, 2, 3, 4) purification with the E-Z 96™ Cycle Pure Kit. Samples were analyzed on a 1% TAE precast agarose gel (MA7351). M: High throughput ladder I (M04-01).