The E-Z 96™ Total RNA Kit is designed for isolation of total cellular RNA from up to 5 x 105 cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilizing HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High-quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96™ Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.
For Research Use Only. Not for use in diagnostic procedures.
FEATURES | SPECIFICATIONS |
---|---|
Downstream Application | PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification |
Elution Volume | 50-75 μL |
Starting Material | Culture cells |
Starting Amount | up to 5×105 cells |
Processing Mode | Manual, centrifugation/vacuum |
Throughput | 96 samples per run |
RNA Binding technology | Silica membrane 96-well |
Figure 1. Purified total RNA from 10 mg chicken liver was isolated with the E-Z 96™ Total RNA Kit. Total RNA (500 ng/lane) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.
Product Information
Safety Data Sheets
Components | Hazard Standards | Languages | Link | hf:tax:dlp_document_language | hf:tax:dlp_document_hazard-standard |
---|---|---|---|---|---|
Nuclease-free Water | GHS | Spanish | spanish | ghs | |
Nuclease-free Water | REACH | Greek | greek | reach | |
Nuclease-free Water | REACH | Portuguese | portuguese | reach | |
Nuclease-free Water | REACH | Hungarian | hungarian | reach | |
Nuclease-free Water | REACH | Dutch | dutch | reach | |
Nuclease-free Water | WHMS | French | french | whms | |
Nuclease-free Water | WHMS | English | english | whms | |
Nuclease-free Water | REACH | Swedish | swedish | reach | |
Nuclease-free Water | REACH | Spanish | spanish | reach | |
Nuclease-free Water | REACH | Italian | italian | reach | |
Nuclease-free Water | GHS | English | english | ghs | |
Nuclease-free Water | REACH | English | english | reach | |
Nuclease-free Water | REACH | Danish | danish | reach | |
Nuclease-free Water | REACH | French | french | reach | |
Nuclease-free Water | REACH | Norwegian | norwegian | reach | |
Nuclease-free Water | REACH | German | german | reach | |
Nuclease-free Water | REACH | Finnish | finnish | reach | |
RNA Wash Buffer II | GHS | English | english | ghs | |
RNA Wash Buffer II | WHMS | French | french | whms | |
RNA Wash Buffer II | WHMS | English | english | whms | |
RNA Wash Buffer II | REACH | Swedish | swedish | reach | |
RNA Wash Buffer II | REACH | Spanish | spanish | reach | |
RNA Wash Buffer II | REACH | Norwegian | norwegian | reach | |
RNA Wash Buffer II | REACH | German | german | reach | |
RNA Wash Buffer II | GHS | Spanish | spanish | ghs | |
RNA Wash Buffer II | REACH | English | english | reach | |
RNA Wash Buffer II | REACH | Danish | danish | reach | |
RNA Wash Buffer II | REACH | French | french | reach | |
RNA Wash Buffer II | REACH | Italian | italian | reach | |
RNA Wash Buffer II | REACH | Finnish | finnish | reach | |
RWF Buffer | GHS | Spanish | spanish | ghs | |
RWF Buffer | WHMS | French | french | whms | |
RWF Buffer | WHMS | English | english | whms | |
RWF Buffer | REACH | Swedish | swedish | reach | |
RWF Buffer | REACH | Spanish | spanish | reach | |
RWF Buffer | REACH | Norwegian | norwegian | reach | |
RWF Buffer | REACH | German | german | reach | |
RWF Buffer | GHS | English | english | ghs | |
RWF Buffer | REACH | English | english | reach | |
RWF Buffer | REACH | Danish | danish | reach | |
RWF Buffer | REACH | Finnish | finnish | reach | |
RWF Buffer | REACH | French | french | reach | |
RWF Buffer | REACH | Italian | italian | reach | |
TRK Lysis Buffer | GHS | English | english | ghs | |
TRK Lysis Buffer | GHS | Spanish | spanish | ghs | |
TRK Lysis Buffer | REACH | English | english | reach | |
TRK Lysis Buffer | REACH | Danish | danish | reach | |
TRK Lysis Buffer | REACH | Finnish | finnish | reach | |
TRK Lysis Buffer | REACH | French | french | reach | |
TRK Lysis Buffer | REACH | German | german | reach | |
TRK Lysis Buffer | REACH | Italian | italian | reach | |
TRK Lysis Buffer | REACH | Norwegian | norwegian | reach | |
TRK Lysis Buffer | REACH | Spanish | spanish | reach | |
TRK Lysis Buffer | REACH | Swedish | swedish | reach | |
TRK Lysis Buffer | WHMS | English | english | whms | |
TRK Lysis Buffer | WHMS | French | french | whms |