E.Z.N.A.® Bacterial DNA Kit

$0.00$568.80

Isolate DNA from gram-positive and negative bacteria using spin columns

  • Reliable – Reproducible DNA purification from a variety of sample sources
  • High yield – Glass beads and enzymatic digestion for cell lysis

The E.Z.N.A.® Bacterial DNA Kit allows the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of gram-positive and negative bacterial species. This kit uses an optimized lysis condition and up to 1 x 10^9 bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or Elution Buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting Amount1 x 109 cells
Starting MaterialBacterial cells
Yield30 µg
Elution Volume50-100 μL
TechnologyHiBind® DNA Mini Column
Processing ModeManual
Throughput1-24

Figure 1. Genomic DNA purified using the E.Z.N.A.® Bacterial DNA Kit. Purified genomic DNA from various bacteria were isolated with the E.Z.N.A.® Bacterial DNA Kit. DNA was extracted in duplicate from each of the following samples: sample 1 from S. aureus, sample 2 from B. subtilis and sample 3 from P. aeruginosa. Genomic DNA (5% of total purified DNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the DNA.

Product Information

NameDocumentLanguagesLink
Quick GuideProduct ManualEnglish
Protocol ManualProduct ManualEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
BL BufferGHSEnglishenglishghs
BL BufferGHSSpanishspanishghs
BL BufferREACHEnglishenglishreach
BL BufferREACHDanishdanishreach
BL BufferREACHFinnishfinnishreach
BL BufferREACHFrenchfrenchreach
BL BufferREACHGermangermanreach
BL BufferREACHItalianitalianreach
BL BufferREACHNorwegiannorwegianreach
BL BufferREACHSpanishspanishreach
BL BufferREACHSwedishswedishreach
BL BufferWHMSEnglishenglishwhms
BL BufferWHMSFrenchfrenchwhms
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferWHMSFrenchfrenchwhms
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
HBC BufferGHSEnglishenglishghs
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHGermangermanreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHSwedishswedishreach
HBC BufferWHMSEnglishenglishwhms
HBC BufferWHMSFrenchfrenchwhms
LysozymeGHSEnglishenglishghs
LysozymeGHSSpanishspanishghs
LysozymeREACHEnglishenglishreach
LysozymeREACHDanishdanishreach
LysozymeREACHFinnishfinnishreach
LysozymeREACHFrenchfrenchreach
LysozymeREACHGermangermanreach
LysozymeREACHItalianitalianreach
LysozymeREACHNorwegiannorwegianreach
LysozymeREACHSpanishspanishreach
LysozymeREACHSwedishswedishreach
LysozymeWHMSEnglishenglishwhms
LysozymeWHMSFrenchfrenchwhms
Proteinase K SolutionGHSEnglishenglishghs
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHFinnishfinnishreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionREACHNorwegiannorwegianreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHPortugueseportuguesereach
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHFrenchfrenchreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
TL BufferGHSEnglishenglishghs
TL BufferGHSSpanishspanishghs
TL BufferREACHEnglishenglishreach
TL BufferREACHDanishdanishreach
TL BufferREACHFinnishfinnishreach
TL BufferREACHFrenchfrenchreach
TL BufferREACHGermangermanreach
TL BufferREACHItalianitalianreach
TL BufferREACHNorwegiannorwegianreach
TL BufferREACHSpanishspanishreach
TL BufferREACHSwedishswedishreach
TL BufferWHMSEnglishenglishwhms
TL BufferWHMSFrenchfrenchwhms
TL BufferREACHDutchdutchreach
TL BufferREACHHungarianhungarianreach
TL BufferREACHPortugueseportuguesereach
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