E.Z.N.A.® Bacterial DNA Kit
Price range: $0.00 through $614.60
Isolate DNA from gram-positive and negative bacteria using spin columns
- Reliable – Reproducible DNA purification from a variety of sample sources
- High yield – Glass beads and enzymatic digestion for cell lysis
The E.Z.N.A.® Bacterial DNA Kit allows the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of gram-positive and negative bacterial species. This kit uses an optimized lysis condition and up to 1 x 10^9 bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or Elution Buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Starting Amount | 1 x 109 cells |
| Starting Material | Bacterial cells |
| Yield | 30 µg |
| Elution Volume | 50-100 μL |
| Technology | HiBind® DNA Mini Column |
| Processing Mode | Manual |
| Throughput | 1-24 |
Figure 1. Genomic DNA purified using the E.Z.N.A.® Bacterial DNA Kit. Purified genomic DNA from various bacteria were isolated with the E.Z.N.A.® Bacterial DNA Kit. DNA was extracted in duplicate from each of the following samples: sample 1 from S. aureus, sample 2 from B. subtilis and sample 3 from P. aeruginosa. Genomic DNA (5% of total purified DNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the DNA.
High DNA Yield and Purity
Figure 2. Purified genomic DNA from B. subtilis was isolated with the E.Z.N.A.® Bacterial DNA Kit and eluted in 100 µL. DNA concentration was quantified via Thermo Scientific’s NanoDrop® 2000c. A260/A280 ratios were approximately 1.8 suggesting high purity for DNA.
High Integrity and Minimal Degradation
Figure 3. Purified genomic DNA from B. subtilis was isolated with the E.Z.N.A.® Bacterial DNA Kit and eluted in 100 µL. 5 µL of eluate was visualized on a 1% agarose gel. Intact, high-molecular-weight bacterial genomic DNA can be seen demonstrating excellent extraction quality.
Inhibitor-Free DNA
Figure 4. Purified genomic DNA from B. subtilis was isolated with the E.Z.N.A.® Bacterial DNA Kit and eluted in 100 µL. qPCR results for gDNA extracted using the E.Z.N.A.® Bacterial DNA Kit demonstrate an expected ΔCt of ~3.3, reflecting minimal PCR inhibition.