E.Z.N.A.® Bacterial RNA Kit
$0.00 – $239.20
Isolate high-quality RNA from gram-positive or gram-negative bacterial species using silica spin columns.
The E.Z.N.A.® Bacterial RNA Kit is designed for the isolation of high-quality total RNA from a variety of bacterial strains. Up to 1 x 109 log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridization techniques.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification |
| Elution Volume | 50-100 μL |
| Starting Material | Bacteria |
| Starting Amount | Up to 3 mL culture, 1 x 109 cells |
| RNA Yield | up to 100 µg |
| Processing Mode | Manual, centrifugation/vacuum |
| Throughput | 1 – 24 |
| RNA Binding Technology | Silica Mini spin column |
| Binding Capacity | 100 µg |
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| HiBind® RNA Mini Columns | — |
| 2 mL Collection Tubes | View Product |
| OTRK Lysis Buffer | — |
| RNA Wash Buffer I | View Product |
| RNA Wash Buffer II | View Product |
| Nuclease-free Water | — |
| Lysozyme | — |
| Glass Beads S | — |
Yield and Quality
Figure 1. Total RNA purified using the E.Z.N.A.® Bacterial RNA Kit. RNA was extracted from 2 mL bacterial samples as follows: sample 1 from Pseudomonas arginosa, sample 2 from Enterococcus faecalis, and sample 3 from Staphylococcus aureus. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.
Yield and Quality from E. coli
Figure 2. Total RNA purified using the E.Z.N.A.® Bacterial RNA Kit. RNA was extracted from lag phase E. coli cultures as follows: sample 1 from 0.5 mL culture, sample 2 from 1.0 mL culture, sample 3 from 2 mL culture, and sample 4 from 3 mL culture. Lanes 1-4 represent the first 100 µL elution. Total RNA (50% of total purified RNA) was analyzed on a denaturing formaldehyde agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.
