E.Z.N.A.® FFPE DNA Kit

$0.00$171.40

Isolate DNA from FFPE samples using spin columns

The E.Z.N.A.® FFPE DNA Kit is designed for fast and easy purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Paraffin removal can be performed using a xylene and ethanol method or efficient heat treatment. Samples are incubated in a specialized lysis buffer along with Proteinase K to reverse crosslinking, effectively releasing short and long DNA fragments. After adjusting the binding conditions with ethanol, the lysate is applied to the MicroElute® DNA column to bind DNA. Cellular debris and proteins are effectively removed during the wash steps. High quality purified DNA is then eluted in elution buffer or water and is ready for applications such as PCR and next-generation sequencing.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting Amount3-8 paraffin sections 5-10 µm thick
Starting MaterialFFPE tissue
Elution Volume50-75 µL
TechnologyMicroElute® LE DNA Column
Processing ModeManual
Throughput1-24

Figure 1. Quality and yield of DNA with agarose gel analysis comparing Company A’s FFPE Kit and Omega Bio-tek’s E.Z.N.A.® FFPE DNA Kit. Analysis was completed on a 0.5% agarose gel. Lanes 1-3 represent Company A and lanes 4-6 represent Omega Bio-tek’s E.Z.N.A.® FFPE DNA Kit. M: 1 kb marker. FFPE DNA tissue starting material was ~2 mg post-deparaffinization. An overnight digestion was completed at 55°C. Samples were eluted in 60 µL elution buffer. O.D. ratio and yield calculated using a Thermo Fisher NanoDrop® 2000c.

Product Information

NameDocumentLanguagesLink
Quick GuideProduct ManualEnglish
Protocol ManualProduct ManualEnglish
E.Z.N.A.® FFPE DNA KitProduct LiteratureEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
BL BufferGHSSpanishspanishghs
BL BufferGHSEnglishenglishghs
BL BufferWHMSFrenchfrenchwhms
BL BufferWHMSEnglishenglishwhms
BL BufferREACHSwedishswedishreach
BL BufferREACHSpanishspanishreach
BL BufferREACHNorwegiannorwegianreach
BL BufferREACHItalianitalianreach
BL BufferREACHGermangermanreach
BL BufferREACHFrenchfrenchreach
BL BufferREACHFinnishfinnishreach
BL BufferREACHDanishdanishreach
BL BufferREACHEnglishenglishreach
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHGermangermanreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHDutchdutchreach
Elution BufferWHMSFrenchfrenchwhms
Elution BufferWHMSEnglishenglishwhms
Elution BufferREACHSwedishswedishreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHGermangermanreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHEnglishenglishreach
Elution BufferGHSSpanishspanishghs
Elution BufferGHSEnglishenglishghs
FTL2 BufferGHSEnglishenglishghs
FTL2 BufferWHMSFrenchfrenchwhms
FTL2 BufferWHMSEnglishenglishwhms
FTL2 BufferREACHSwedishswedishreach
FTL2 BufferREACHSpanishspanishreach
FTL2 BufferREACHNorwegiannorwegianreach
FTL2 BufferREACHItalianitalianreach
FTL2 BufferREACHFrenchfrenchreach
FTL2 BufferREACHFinnishfinnishreach
FTL2 BufferREACHDanishdanishreach
FTL2 BufferREACHEnglishenglishreach
FTL2 BufferGHSSpanishspanishghs
FTL2 BufferREACHGermangermanreach
HBC BufferWHMSEnglishenglishwhms
HBC BufferWHMSFrenchfrenchwhms
HBC BufferREACHSwedishswedishreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHEnglishenglishreach
HBC BufferGHSSpanishspanishghs
HBC BufferGHSEnglishenglishghs
HBC BufferREACHGermangermanreach
Proteinase K SolutionREACHPortugueseportuguesereach
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHNorwegiannorwegianreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHFinnishfinnishreach
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionGHSEnglishenglishghs
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