E.Z.N.A.® Food DNA Kit

$0.00$181.40

Isolate DNA from host and pathogens in food samples using spin columns.

Minus Quantity- Plus Quantity+

The E.Z.N.A.® Food DNA Kit allows rapid and reliable isolation of high-quality DNA from complex matrices such as processed food, chocolate, cereals, and meat. Specific protocols exist for target host DNA for GMO testing or bacterial DNA for pathogen/spoilation testing. Omega Bio-tek’s MB1 Buffer allows for efficient homogenization of samples without foaming commonly seen in lysis buffers containing detergents resulting in higher yields.

The system combines the Omega Bio-tek’s HiBind® DNA Mini Columns with RBB Buffer to eliminate PCR inhibiting compounds within the samples and elute highly concentrated DNA. The purified DNA can be used in PCR-based testing for genetically modified organisms (GMO) DNA. There are no organic extractions thus reducing plastic waste and hands-on time and multiple samples can be processed in parallel.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting Amount200 mg
Starting MaterialProcessed food, chocolate, cereals, and meat
Yield30 µg
Elution Volume50-100 μL
TechnologyHiBind® DNA Mini Column
Processing ModeManual
Throughput1-24
ITEMAVAILABLE SEPARATELY
HiBind® DNA Mini ColumnsView Product
2 mL Collection TubesView Product
MB1 Buffer
DS Buffer
RBB BufferView Product
HBC BufferView Product
DNA Wash BufferView Product
Proteinase K SolutionView Product
Elution BufferView Product

Yield

Figure 1. DNA was extracted from 20 mg samples as per manufacturer’s recommended protocols and eluted in 100 µL. A 20 µL SYBR® Green real-time PCR was performed in triplicate using 2 µL eluate as the template.

qPCR Comparison

Figure 2. DNA was extracted from 200 mg samples as per manufacturer’s recommended protocols and eluted in 100 µL. A 20 µL SYBR® Green real-time PCR was performed in triplicate using 2 µL eluate as the template.

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