E.Z.N.A.® Micro RNA Kit
$0.00 – $328.70
Isolation of miRNA & large RNA in separate fractions using spin columns
The E.Z.N.A.® Micro RNA Kit combines the reversible binding properties of the HiBind® matrix, a new silica-based material, with the highly efficient lysis ability of RNA-Solv® to extract micro and large (>200 nt) RNA from a wide variety of starting materials. Micro and large RNA are separated to allow for quantification into downstream applications.
The system uses RNA-Solv® to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is applied to a HiBind® RNA column where large RNA binds. The flow-through is retained and applied to a HiBind® Micro RNA column to bind small RNA.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | RTPCR*, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation |
| Elution Volume | 15-30 μL for miRNA; 40-70 μL for >200 nt |
| Starting Material | Cultured eukaryotic cells or bacteria, or from animal, plant, or fungal tissues |
| Starting Amount | Up to 100 mg |
| RNA Yield | Up to 50 µg |
| Processing Mode | Spin column |
| Throughput | 1 – 24 |
| RNA Binding Technology | Silica technology |
| Binding Capacity | 100 µg for each |
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| HiBind® Micro RNA Column | — |
| HiBind® RNA Mini Column | — |
| 2 mL Collection Tubes | View Product |
| RNA-Solv® | View Product |
| RNA Wash Buffer I | View Product |
| RNA Wash Buffer II | View Product |
| Nuclease-free Water | — |
Figure 1. Purified large RNAs and miRNA from tissue samples was isolated with the E.Z.N.A.® miRNA Isolation Kit. RNA was extracted from samples as follows: sample 1 from fish liver, sample 2 from frog liver. Large RNA (5% of total purified RNA) was analyzed on a 1% agarose gel. miRNA (5% of total purified RNA) was analyzed on a 3% agarose gel to demonstrate yield and quality of the RNA.
