E.Z.N.A.® MicroElute® Cycle Pure Kit
- Elution volumes – 10-15µl
- Rapid – Purification of PCR products in 10 minutes
- Convenient – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for downstream applications
This product has been replaced with the MicroElute® Cycle-Pure & Gel Extraction Kit.
Modified from the E.Z.N.A.® Cycle Pure Kit, the E.Z.N.A.® MicroElute® Cycle Pure Kit is specially designed to purify PCR samples with a small elution volume of 10-15 µL. Highly concentrated DNA eluates are ready to use in all subsequent applications.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream application | Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| Elution volume | 10-15 µL |
| Starting material | PCR reaction |
| Starting amount | Up to 10 µg DNA |
| DNA recovered | >90% recovery, 70 bp to 20 kb |
| Processing mode | Manual, centrifugation/vacuum |
| Throughput | 1 |
| DNA binding technology | Silica MicroElute spin column |
| Binding capacity | 10 μg |
| Processing time | <15 minutes |
| Special note | Low elution volume |
Figure 1. Effect of elution volume on concentration and recovery using the MicroElute® Cycle Pure Kit. Elution volume versus recovery and DNA yield; 11 µg of 3 kb PCR product was purified using the MicroElute® Cycle Pure protocol and eluted with the indicated volume of elution buffer. The standard protocol uses 15 µL elution buffer because this combines higher yield and higher concentration. The yield can be increased by increasing the elution volume.