E.Z.N.A.® Plant RNA Kit

$0.00$1,059.40

Isolate total RNA from plant samples using spin columns

Minus Quantity- Plus Quantity+

E.Z.N.A.® Plant RNA Kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. This kit provides a homogenizer column for filtration and homogenization of viscous plant cell lysate by centrifugation in combination with the HiBind RNA spin column for RNA purification. All the contaminants including polysaccharides and phenolic compounds are effectively removed. Purified RNA can be used for most downstream applications such as RT-PCR, Northern blot analysis, differential display, and poly A+ RNA selection.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Downstream ApplicationPCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume50-100 μL
Starting MaterialPlant tissue
Starting Amount<100 mg
RNA YieldUp to 100 µg, >200 nt
Processing ModeSpin column
Throughput1 – 24
RNA Binding TechnologySilica technology
Binding Capacity100 µg
ITEMAVAILABLE SEPARATELY
HiBind® RNA Mini Columns
2 mL Collection TubesView Product
Homogenizer Mini Columns
NTL Lysis Buffer
SP Buffer
RB Buffer
RNA Wash Buffer IView Product
RNA Wash Buffer IIView Product
Nuclease-free Water

Figure 1. Omega Bio-tek’s yield vs. Company Q’s yield. Total RNA was isolated from 100 mg of Arabidopsis thaliana leaf tissue according to manufacturer’s recommended protocols. One tenth of eluate was analyzed on a denaturing agent agarose gel. O.D. values calculated with Thermo Scientific’s NanoDrop® 2000c.

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