E.Z.N.A.® Plant & Fungal RNA Kit

The E.Z.N.A.® RNA family of products is an innovative system that radically simplifies the extraction and purification of RNA from a variety of sources. The key to this system is that it uses the reversible binding properties of the HiBind® matrix (a silica-based material) in combination with the speed of mini column spin technology. Single or multiple samples can be processed quickly. There is no need for phenol/chloroform extractions and time-consuming steps, such as CsCl gradient ultra-centrifugation and precipitation with isopropanol or LiCL, are eliminated.

RNA purified using the E.Z.N.A.® RNA purification system is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

The E.Z.N.A.® Plant & Fungal RNA Kit can purify up to 100 µg plant or fungal RNA that is >200 nt. Normally, 10-100 mg plant or fungal tissue can be processed in a single experiment. Lysis of cells or tissue occurs under denaturing conditions that inactivate RNases. After the homogenization process, samples are transferred to the HiBind® RNA Mini Column to bind RNA. Cellular debris and other contaminants are removed after three quick wash steps. High-quality RNA is eluted in sterile Nuclease-free Water.