E.Z.N.A.® Plasmid DNA Mini Kit II

Price range: $0.00 through $311.00

  • Rapid – Purification of plasmid DNA in 30 minutes or less
  • Safe – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for downstream applications
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This second-generation plasmid miniprep kit yields 40-70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid DNA Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 mL to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Downstream ApplicationCloning; sanger sequencing etc.
Starting material5-15 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeManual, centrifugation or vacuum
Throughput1 – 24
DNA binding technologySilica Mini Spin Column II (2x capacity of mini spin column)
Lysate clearance methodCentrifugation
Processing time<30 minutes
Yield40-70 µg for high copy-number; 1-15 µg for low copy-number

High-Quality Plasmid Extractions

Figure 1. pGEM and pGL4.51 plasmids were purified from 15 mL DH5α cultures and eluted in 100 µL using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Mini Kit II. Plasmid DNA yields were determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system. All samples have ideal A260/A280 (~1.8) and A260/A230 (~2.0) absorbance ratios, indicating high-quality purified DNA.

Unfragmented Plasmid DNA Purification

Figure 2. 200 ng purified pGEM and pGL4.51 plasmid were analyzed on a 0.8% Agarose gel. Plasmids were isolated from 15 mL DH5α cultures using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Mini Kit II. Plasmids were digested with HF-Scal to linearize.

Sequencing-Quality Plasmid Purification

Plasmid Type Sample Trace Score Continuous Read Length QV20+
pGEM 1 57 962 969
2 56 953 957
3 56 963 965
pGL4.51 1 57 970 978
2 57 973 978
3 53 951 935

Table 1. pGEM and pGL4.51 plasmids were purified from 15 mL DH5α cultures harboring plasmids and eluted in 100 µL using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Mini Kit II. The plasmids were then used for Sanger Sequencing.  All samples display high continuous read length and QV20+ scores, indicating plasmids of high-quality isolated from this kit.

Product Information

Document NameLanguagesLink
Protocol ManualEnglish
Quick GuideEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferREACHLatvianlatvianreach
DNA Wash BufferREACHPolishpolishreach
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHGreekgreekreach
Elution BufferREACHLatvianlatvianreach
Elution BufferREACHPolishpolishreach
HBC BufferGHSEnglishenglishghs
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHGermangermanreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHSwedishswedishreach
HBC BufferWHMSEnglishenglishwhms
HBC BufferWHMSFrenchfrenchwhms
HBC BufferREACHLatvianlatvianreach
HBC BufferREACHPolishpolishreach
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHFrenchfrenchreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
RNase AREACHLatvianlatvianreach
RNase AREACHPolishpolishreach
Solution IGHSEnglishenglishghs
Solution IGHSSpanishspanishghs
Solution IREACHEnglishenglishreach
Solution IREACHDanishdanishreach
Solution IREACHFinnishfinnishreach
Solution IREACHFrenchfrenchreach
Solution IREACHGermangermanreach
Solution IREACHItalianitalianreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHSpanishspanishreach
Solution IREACHSwedishswedishreach
Solution IWHMSEnglishenglishwhms
Solution IWHMSFrenchfrenchwhms
Solution IREACHLatvianlatvianreach
Solution IREACHPolishpolishreach
Solution IIGHSEnglishenglishghs
Solution IIGHSSpanishspanishghs
Solution IIREACHEnglishenglishreach
Solution IIREACHDanishdanishreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHFrenchfrenchreach
Solution IIREACHGermangermanreach
Solution IIREACHItalianitalianreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSpanishspanishreach
Solution IIREACHSwedishswedishreach
Solution IIWHMSEnglishenglishwhms
Solution IIWHMSFrenchfrenchwhms
Solution IIREACHLatvianlatvianreach
Solution IIREACHPolishpolishreach
Solution IIIGHSEnglishenglishghs
Solution IIIGHSSpanishspanishghs
Solution IIIREACHEnglishenglishreach
Solution IIIREACHDanishdanishreach
Solution IIIREACHFinnishfinnishreach
Solution IIIREACHFrenchfrenchreach
Solution IIIREACHGermangermanreach
Solution IIIREACHItalianitalianreach
Solution IIIREACHNorwegiannorwegianreach
Solution IIIREACHSpanishspanishreach
Solution IIIREACHSwedishswedishreach
Solution IIIWHMSEnglishenglishwhms
Solution IIIWHMSFrenchfrenchwhms
Solution IIIREACHLatvianlatvianreach
Solution IIIREACHPolishpolishreach
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