E.Z.N.A.® Plasmid DNA Mini Kit II
$0.00 – $311.00
- Rapid – Purification of plasmid DNA in 30 minutes or less
- Safe – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for downstream applications
This second-generation plasmid miniprep kit yields 40-70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid DNA Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 mL to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | Cloning; sanger sequencing etc. |
| Starting material | 5-15 mL LB culture with OD600 between 2 and 3; or equivalent |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing mode | Manual, centrifugation or vacuum |
| Throughput | 1 – 24 |
| DNA binding technology | Silica Mini Spin Column II (2x capacity of mini spin column) |
| Lysate clearance method | Centrifugation |
| Processing time | <30 minutes |
| Yield | 40-70 µg for high copy-number; 1-15 µg for low copy-number |