E.Z.N.A.® Plasmid DNA Mini Kit II
Price range: $0.00 through $326.20
- Rapid – Purification of plasmid DNA in 30 minutes or less
- Safe – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for downstream applications
This second-generation plasmid miniprep kit yields 40-70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid DNA Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 mL to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | Cloning; sanger sequencing etc. |
| Starting material | 5-15 mL LB culture with OD600 between 2 and 3; or equivalent |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing mode | Manual, centrifugation or vacuum |
| Throughput | 1 – 24 |
| DNA binding technology | Silica Mini Spin Column II (2x capacity of mini spin column) |
| Lysate clearance method | Centrifugation |
| Processing time | <30 minutes |
| Yield | 40-70 µg for high copy-number; 1-15 µg for low copy-number |
High-Quality Plasmid Extractions
Figure 1. pGEM and pGL4.51 plasmids were purified from 15 mL DH5α cultures and eluted in 100 µL using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Mini Kit II. Plasmid DNA yields were determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system. All samples have ideal A260/A280 (~1.8) and A260/A230 (~2.0) absorbance ratios, indicating high-quality purified DNA.
Unfragmented Plasmid DNA Purification
Figure 2. 200 ng purified pGEM and pGL4.51 plasmid were analyzed on a 0.8% Agarose gel. Plasmids were isolated from 15 mL DH5α cultures using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Mini Kit II. Plasmids were digested with HF-Scal to linearize.
Sequencing-Quality Plasmid Purification
| Plasmid Type | Sample | Trace Score | Continuous Read Length | QV20+ |
|---|---|---|---|---|
| pGEM | 1 | 57 | 962 | 969 |
| 2 | 56 | 953 | 957 | |
| 3 | 56 | 963 | 965 | |
| pGL4.51 | 1 | 57 | 970 | 978 |
| 2 | 57 | 973 | 978 | |
| 3 | 53 | 951 | 935 |
Table 1. pGEM and pGL4.51 plasmids were purified from 15 mL DH5α cultures harboring plasmids and eluted in 100 µL using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Mini Kit II. The plasmids were then used for Sanger Sequencing. All samples display high continuous read length and QV20+ scores, indicating plasmids of high-quality isolated from this kit.