Mag-Bind® Blood DNA HV Kit CE IVD
Updated in 2024!
CE-IVD marked – for ordering information, please fill out the form below
Isolate DNA from up to 4 mL whole blood using magnetic beads
The Mag-Bind® Blood DNA HV Kit CE IVD is intended for isolation and purification of genomic DNA from up to 4 mL whole blood samples.
The Mag-Bind® Blood DNA HV Kit CE IVD utilizes magnetic bead-based technology and can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors. The Mag-Bind® Blood DNA HV Kit CE IVD can be used to process up to 2 mL whole blood samples on Omega Bio-tek’s MagBinder® Fit24 Nucleic Acid Purification System when user-filled into MagBinder compatible cartridges.
| FEATURES | SPECIFICATIONS |
|---|---|
| Starting Amount | Up to 4 mL |
| Starting Material | Whole blood |
| Elution Volume | 0.4 – 1 mL |
| Technology | Magnetic beads |
| Processing Mode | Automated, Manual |
| Throughput | 24 |
| Note | Recovered DNA exhibits good purity with A260/A280 = ~1.8 and A260/A230 > 1.8. |
High-quality DNA with excellent purity ratios
| Sample | DNA Yield (µg) | A260/A280 | A260/A230 | |
| NanoDrop | QuantiFluor System | |||
| 1 | 119.2 | 112 | 1.87 | 1.93 |
| 2 | 120.1 | 108 | 1.86 | 2.12 |
| 3 | 114.9 | 114 | 1.86 | 1.98 |
Table 1. DNA was manually extracted from 4 mL whole blood samples and was eluted in 1 mL volume. Purified DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system and Promega’s QuantiFluor® dsDNA system. The concordant readings indicate the DNA purified is primarily dsDNA.
qPCR Analysis of Purified DNA
Figure 1. Average Ct values obtained using human-specific primers on 10X and 100X dilutions of purified DNA used as the template. The Ct values differed by ~ 3 cycles per 10-fold dilution indicating inhibitor-free DNA.
DNA Yield from 2 mL whole blood samples purified on MagBinder Fit24 vs Manual
Figure 2. Average DNA Yield from 2 mL sample volume from three different lots of blood extracted manually vs automated on MagBinder® Fit24 Nucleic Acid Purification System. The DNA yield was determined using Thermo Scientific’s NanoDrop 2000c and found to be comparable between the manual and automated DNA extractions.
High Molecular Weight DNA extracted from blood samples
Figure 3. TapeStation® Analysis of Purified DNA. TapeStation® analysis of genomic DNA purified from 2 mL of whole blood samples using Omega Bio-tek’s Mag-Bind® Blood DNA HV Kit processed manually and on MagBinder® Fit24 Nucleic Acid Purification System.