Mag-Bind® Blood DNA HV Kit CE IVD

Updated in 2024!

CE-IVD marked – for ordering information, please fill out the form below

Isolate DNA from up to 4 mL whole blood using magnetic beads

The Mag-Bind® Blood DNA HV Kit CE IVD is intended for isolation and purification of genomic DNA from up to 4 mL whole blood samples.

The Mag-Bind® Blood DNA HV Kit CE IVD utilizes magnetic bead-based technology and can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors. The Mag-Bind® Blood DNA HV Kit CE IVD can be used to process up to 2 mL whole blood samples on Omega Bio-tek’s MagBinder® Fit²⁴ Nucleic Acid Purification System when user-filled into MagBinder compatible cartridges.

FEATURES	SPECIFICATIONS
Starting Amount	Up to 4 mL
Starting Material	Whole blood
Elution Volume	0.4 – 1 mL
Technology	Magnetic beads
Processing Mode	Automated, Manual
Throughput	24
Note	Recovered DNA exhibits good purity with A260/A280 = ~1.8 and A260/A230 > 1.8.

High-quality DNA with excellent purity ratios

*Sample*	DNA Yield (µg)		A260/A280	A260/A230
*Sample*	NanoDrop	QuantiFluor System	A260/A280	A260/A230
1	119.2	112	1.87	1.93
2	120.1	108	1.86	2.12
3	114.9	114	1.86	1.98

Table 1. DNA was manually extracted from 4 mL whole blood samples and was eluted in 1 mL volume. Purified DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system and Promega’s QuantiFluor® dsDNA system. The concordant readings indicate the DNA purified is primarily dsDNA.

qPCR Analysis of Purified DNA

Figure 1. Average Ct values obtained using human-specific primers on 10X and 100X dilutions of purified DNA used as the template. The Ct values differed by ~ 3 cycles per 10-fold dilution indicating inhibitor-free DNA.

DNA Yield from 2 mL whole blood samples purified on MagBinder Fit²⁴ vs Manual

Figure 2. Average DNA Yield from 2 mL sample volume from three different lots of blood extracted manually vs automated on MagBinder® Fit²⁴ Nucleic Acid Purification System. The DNA yield was determined using Thermo Scientific’s NanoDrop 2000c and found to be comparable between the manual and automated DNA extractions.

High Molecular Weight DNA extracted from blood samples

Figure 3. TapeStation® Analysis of Purified DNA. TapeStation® analysis of genomic DNA purified from 2 mL of whole blood samples using Omega Bio-tek’s Mag-Bind® Blood DNA HV Kit processed manually and on MagBinder® Fit²⁴ Nucleic Acid Purification System.

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Mag-Bind® Blood DNA HV Kit CE IVD

High-quality DNA with excellent purity ratios

qPCR Analysis of Purified DNA

DNA Yield from 2 mL whole blood samples purified on MagBinder Fit²⁴ vs Manual

High Molecular Weight DNA extracted from blood samples

LET’S GET STARTED – TELL US MORE ABOUT YOUR PROJECT:

Product Information

Safety Data Sheets

Mag-Bind® Blood DNA HV Kit CE IVD

High-quality DNA with excellent purity ratios

qPCR Analysis of Purified DNA

DNA Yield from 2 mL whole blood samples purified on MagBinder Fit24 vs Manual

High Molecular Weight DNA extracted from blood samples

LET’S GET STARTED – TELL US MORE ABOUT YOUR PROJECT:

Product Information

Safety Data Sheets

DNA Yield from 2 mL whole blood samples purified on MagBinder Fit²⁴ vs Manual