The M3760 Mag-Bind® cfNA Kit is a magnetic bead-based nucleic acid purification system designed for the efficient isolation of both cell-free DNA (cfDNA) and cell-free RNA (cfRNA) from plasma/serum samples. By enabling the recovery of two critical analytes within a streamlined workflow, the kit supports a wide range of liquid biopsy and translational research applications, including oncology research, biomarker discovery, prenatal research, and next-generation sequencing (NGS) based studies.

The kit is designed to process 1 – 4 mL of plasma or serum per sample, making it suitable for applications requiring enhanced sensitivity through larger sample inputs.

• Samples are first treated with Proteinase K and a Binding Enhancer in a buffered environment.
• Binding buffer master mixed with Mag-Bind® Particles MCR and isopropanol is then added to facilitate binding of cfDNA and cfRNA to the magnetic beads.
• The beads are subjected to several wash steps to remove contaminants followed by elution of cfDNA and cfRNA in a single eluate.

The magnetic properties of the Mag-Bind® Particles MCR enable fast magnetic separation, especially during steps involving large volumes. The high-binding capability of the magnetic beads allows for a lower volume of magnetic particles required thereby reducing the elution volume i.e., cfDNA and cfRNA from up to 4 mL plasma or serum can be eluted in just 50 µL. The purified cfDNA and cfRNA are compatible with a variety of downstream molecular biology applications, including quantitative PCR (qPCR), digital PCR (ddPCR), next-generation sequencing (NGS), targeted mutation analysis, gene expression studies, and multi-omics workflows.

The M3760 Mag-Bind® cfNA Kit has been validated for use with samples collected in EDTA blood collection tubes as well as Streck Cell-Free Nucleic Acid BCT® and Streck Nucleic Acid BCT® Plus tubes, providing flexibility for both standard and stabilized blood collection workflows. In addition to manual processing using 15 mL centrifuge tubes, the kit’s magnetic bead-based format is readily adaptable to high-throughput automation platforms, facilitating scalable and fully automated processing using appropriate plasticware improving laboratory efficiency. The workflow can be automated on a variety of open automation systems, including Hamilton®, Dynamic Devices®, KingFisher™, and MagBinder™ Fit24.