Mag-Bind® Endo-free Plasmid Midi Kit

$0.00$984.37

Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 50 mL culture volume.

  • Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
  • High-yielding – Plasmid DNA yield up to 200 μg
  • Rapid – 24 samples can be processed in ~ 70 minutes starting from a bacterial pellet without manual intervention
  • Versatile – Process up to 50 mL bacterial culture with different plasmid types and culture medias
  • Transfection-quality – Endotoxin free plasmid DNA with endotoxins levels < 0.1 EU/µg
  • Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

The Mag-Bind® Endo-free Plasmid Midi Kit is designed to deliver large scale purification of high-quality plasmid DNA with endotoxins levels <0.1 EU/µg for use in eukaryotic transfections and other sensitive in vitro applications.

The Kit follows magnetic bead-based technology to purify plasmid DNA from up to 50 mL bacterial culture in LB or up to 10 mL bacterial culture in TB or other suitable growth medium. The Mag-Bind® Endo-free Plasmid DNA Kit can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors. Yields may vary depending on the copy number of the plasmid, E. coli strain used, culture media, and condition of growth.

The Mag-Bind® Endo-free Plasmid Midi Kit follows a modified alkaline lysis method forming a bacterial lysate. The lysate is then cleared using either Mag-Bind® Particles LC (sold separately) or Lysate Clearance Filter Syringe (sold separately) or centrifugation. The novel paramagnetic lysate clearance beads bind to cell debris and pull them down when magnetized. This eliminates the need for centrifugation or vacuum manifold for lysate clearance, making this suitable for high-throughput, automation workflows.

Post lysate clearance, plasmid DNA is then bound to Mag-Bind® Particles RQ through a specially formulated binding buffer and the Mag-Bind® particles are subjected to a wash routine to remove endotoxins, salt, and other contaminants. High-quality plasmid DNA is then eluted in Endo-free Water and is ready for use in a wide range of downstream applications such as transfection routine screening, restriction enzyme digestion, transformation, PCR, and DNA sequencing, etc.

For Research Use Only. Not for use in diagnostic procedures.

Feature Specifications
Starting Amount up to 50 mL bacterial culture in LB or up to 10 mL bacterial culture in TB
Starting Material Bacteria harboring high-copy or low-copy plasmid in LB, TB, or other suitable culture medium
Elution Volume 100 µL
Technology Magnetic beads
Processing Mode Automated, Manual
Throughput 1 - 24
Processing Time 24 samples in ~ 70 minutes
Endotoxin levels < 0.1 EU/µg
Purity Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8
Lysate Clearance Methodology Magnetic beads, Centrifugation or Syringe
Downstream Applications Transfection, routine screening, restriction enzyme digestion, transformation, PCR, DNA sequencing, in vitro transcription, and other sensitive applications requiring pure DNA

Included with each kit:

SKU No M1272-00 M1272-01 Available Seperately
Purifications 1 x 24 4 x 24
Solution I 70 mL 270 mL Solution I
Solution II 70 mL 270 mL Solution II
N3 Buffer 60 mL 250 mL
IRD Buffer 100 mL 320 mL IRD Buffer
VHB Buffer 44 mL 176 mL VHB Buffer
Endo-free Water 60 mL 2 x 60 mL
Mag-Bind® Particles RQ 6 mL 22 mL Mag-Bind® Particles RQ
RNase A 300 μL 1.2 mL RNase A

Components for optional protocols:

Product Literature

NameDocumentLanguagesLink
Protocol ManualProduct ManualEnglish
Mag-Bind Endo-free Plasmid Midi KitProduct LiteratureEnglish
Automated Plasmid Purifications using the MagBinder Fit24: Mini, Midi, and MoreApplication NoteEnglish

More convenience with comparable yields vs Company Q

Figure 1. A variety of plasmid types and sizes were purified from DH5α cultures (n=3) using the Mag-Bind® Endo-free Plasmid Midi Kit, in addition to an equivalent kit from Company Q, following respective manufacturers’ recommended protocols. Purified Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system. Plasmid yields were comparable for all plasmid types, irrespective of the extraction kit used. Inset table shows advantages of Omega Bio-tek’s Kit over Company Q’s Kit

Versatility across various Plasmid types and culture media

Figure 2. Mag-Bind® Endo-free Plasmid Midi Kit is capable of handling diverse sample input conditions ranging from plasmid types, plasmid sizes, culture medium, and input culture volumes. The plasmid yield may vary based on these conditions.

Significantly higher Plasmid yields compared to Company T

Figure 3. pGEM and pGL4.51 plasmids were purified from 5 mL DH5α cultures (n=3) using the Mag-Bind® Endo-free Plasmid Midi Kit on the MagBinder® Fit24 Nucleic Acid Purification System, as well as Company T’s Kit following manufacturers’ recommended centrigation protocols. Purified Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system. Plasmid purification was not only endotoxin-free (<0.1 EU/μg) for Omega Bio-tek’s Kit, but the yield was also significantly higher compared to Company T’s Kit.

Higher transfection efficiency vs. Company T

Figure 4. Plasmid pGL4.51 was prepared from 5 mL DH5α cultures (n=3) and purified using the Mag-Bind® Endo-free Plasmid Midi Kit on the MagBinder® Fit24 Nucleic Acid Purification System, as well as Company T’s equivalent Kit. Purified Plasmids were transfected into 10,000 cells with 200 ng of purified plasmid using 2 μL Lipofectamine Transfection Reagent. Plasmids purified using Omega Bio-tek’s Kit resulted in higher bioluminscent signal, indicating higher transfection efficiency compared to plasmids purified using Company T’s Kit.

Product Literature

NameDocumentLanguagesLink
Protocol ManualProduct ManualEnglish
Mag-Bind Endo-free Plasmid Midi KitProduct LiteratureEnglish
Automated Plasmid Purifications using the MagBinder Fit24: Mini, Midi, and MoreApplication NoteEnglish

Product Information

NameDocumentLanguagesLink
Protocol ManualProduct ManualEnglish
Mag-Bind Endo-free Plasmid Midi KitProduct LiteratureEnglish
Automated Plasmid Purifications using the MagBinder Fit24: Mini, Midi, and MoreApplication NoteEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
Endo-free WaterGHSEnglishenglishghs
Endo-free WaterGHSSpanishspanishghs
Endo-free WaterREACHEnglishenglishreach
Endo-free WaterWHMSEnglishenglishwhms
Endo-free WaterWHMSFrenchfrenchwhms
Endo-free WaterREACHDanishdanishreach
Endo-free WaterREACHDutchdutchreach
Endo-free WaterREACHFinnishfinnishreach
Endo-free WaterREACHFrenchfrenchreach
Endo-free WaterREACHGermangermanreach
Endo-free WaterREACHGreekgreekreach
Endo-free WaterREACHHungarianhungarianreach
Endo-free WaterREACHItalianitalianreach
Endo-free WaterREACHNorwegiannorwegianreach
Endo-free WaterREACHPortugueseportuguesereach
Endo-free WaterREACHSpanishspanishreach
Endo-free WaterREACHSwedishswedishreach
IRD BufferGHSEnglishenglishghs
IRD BufferGHSSpanishspanishghs
IRD BufferREACHEnglishenglishreach
IRD BufferWHMSEnglishenglishwhms
IRD BufferWHMSFrenchfrenchwhms
IRD BufferREACHDanishdanishreach
IRD BufferREACHDutchdutchreach
IRD BufferREACHFinnishfinnishreach
IRD BufferREACHFrenchfrenchreach
IRD BufferREACHGermangermanreach
IRD BufferREACHGreekgreekreach
IRD BufferREACHHungarianhungarianreach
IRD BufferREACHItalianitalianreach
IRD BufferREACHNorwegiannorwegianreach
IRD BufferREACHPortugueseportuguesereach
IRD BufferREACHSpanishspanishreach
IRD BufferREACHSwedishswedishreach
Mag-Bind Particles RQGHSEnglishenglishghs
Mag-Bind Particles RQGHSSpanishspanishghs
Mag-Bind Particles RQREACHEnglishenglishreach
Mag-Bind Particles RQREACHDanishdanishreach
Mag-Bind Particles RQREACHFinnishfinnishreach
Mag-Bind Particles RQREACHFrenchfrenchreach
Mag-Bind Particles RQREACHGermangermanreach
Mag-Bind Particles RQREACHItalianitalianreach
Mag-Bind Particles RQREACHNorwegiannorwegianreach
Mag-Bind Particles RQREACHSpanishspanishreach
Mag-Bind Particles RQREACHSwedishswedishreach
Mag-Bind Particles RQWHMSEnglishenglishwhms
Mag-Bind Particles RQWHMSFrenchfrenchwhms
Mag-Bind Particles RQREACHDutchdutchreach
Mag-Bind Particles RQREACHHungarianhungarianreach
Mag-Bind Particles RQREACHPortugueseportuguesereach
Mag-Bind Particles RQREACHGreekgreekreach
N3 BufferGHSEnglishenglishghs
N3 BufferGHSSpanishspanishghs
N3 BufferREACHEnglishenglishreach
N3 BufferREACHDanishdanishreach
N3 BufferREACHFinnishfinnishreach
N3 BufferREACHFrenchfrenchreach
N3 BufferREACHGermangermanreach
N3 BufferREACHItalianitalianreach
N3 BufferREACHNorwegiannorwegianreach
N3 BufferREACHSpanishspanishreach
N3 BufferREACHSwedishswedishreach
N3 BufferWHMSEnglishenglishwhms
N3 BufferWHMSFrenchfrenchwhms
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHFrenchfrenchreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
Solution IGHSEnglishenglishghs
Solution IGHSSpanishspanishghs
Solution IREACHEnglishenglishreach
Solution IREACHDanishdanishreach
Solution IREACHFinnishfinnishreach
Solution IREACHFrenchfrenchreach
Solution IREACHGermangermanreach
Solution IREACHItalianitalianreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHSpanishspanishreach
Solution IREACHSwedishswedishreach
Solution IWHMSEnglishenglishwhms
Solution IWHMSFrenchfrenchwhms
Solution IIGHSEnglishenglishghs
Solution IIGHSSpanishspanishghs
Solution IIREACHEnglishenglishreach
Solution IIREACHDanishdanishreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHFrenchfrenchreach
Solution IIREACHGermangermanreach
Solution IIREACHItalianitalianreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSpanishspanishreach
Solution IIREACHSwedishswedishreach
Solution IIWHMSEnglishenglishwhms
Solution IIWHMSFrenchfrenchwhms
VHB BufferGHSEnglishenglishghs
VHB BufferGHSSpanishspanishghs
VHB BufferREACHEnglishenglishreach
VHB BufferREACHDanishdanishreach
VHB BufferREACHFinnishfinnishreach
VHB BufferREACHFrenchfrenchreach
VHB BufferREACHGermangermanreach
VHB BufferREACHItalianitalianreach
VHB BufferREACHNorwegiannorwegianreach
VHB BufferREACHSpanishspanishreach
VHB BufferREACHSwedishswedishreach
VHB BufferWHMSEnglishenglishwhms
VHB BufferWHMSFrenchfrenchwhms
VHB BufferREACHDutchdutchreach
VHB BufferREACHHungarianhungarianreach
VHB BufferREACHPortugueseportuguesereach
VHB BufferREACHGreekgreekreach
Shopping Cart
Scroll to Top