$0.00 – $630.00
Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 1.5 mL culture volume.
All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.
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Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.
The Mag-Bind® Endo-free Plasmid Mini Kit is designed to deliver high-quality, plasmid DNA with endotoxin levels <0.1 EU/μg for use in eukaryotic transfections and other sensitive in vitro applications.
The kit follows magnetic bead-based technology to purify plasmid DNA from up to 1.5 mL overnight bacterial culture in LB or TB or other suitable growth medium. The Mag-Bind® Endo-free Plasmid Mini Kit can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors. Purified plasmid DNA is endotoxin free (<0.1 EU/μg), and yields may vary depending on the copy number of the plasmid, E. coli strain used, culture media, and conditions of growth.
The Mag-Bind® Endo-free Plasmid Mini Kit follows a modified alkaline lysis method forming a bacterial lysate. The lysate is then cleared using either Mag-Bind® Particles LC (sold separately) or centrifugation. The novel paramagnetic lysate clearance beads bind to cell debris and pulls them down when magnetized. This eliminates the need for centrifugation or vacuum manifold for lysate clearance, making this suitable for high-throughput, automation workflows.
Post lysate clearance, plasmid DNA is then bound to Mag-Bind® Particles RQ through a specially formulated binding buffer and the Mag-Bind® particles are subjected to a wash routine to remove endotoxins, salt, and other contaminants. High-quality plasmid DNA is then eluted in Endo-free Water and is ready for use in a wide range of downstream applications such as transfection, routine screening, restriction enzyme digestion, transformation, PCR, and DNA sequencing etc.
For Research Use Only. Not for use in diagnostic procedures.
| Feature | Specifications |
|---|---|
| Starting Amount | up to 1.5 mL overnight bacterial culture per well of a 96-well deep well plate |
| Starting Material | Bacteria harboring high-copy or low-copy plasmid in LB, TB, or other suitable culture medium |
| Elution Volume | 50 - 100 µL |
| Technology | Magnetic beads |
| Processing Mode | Automated, Manual |
| Throughput | 1 - 96 |
| Processing Time | 96 samples in 95 minutes |
| Endotoxin levels | < 0.1 EU/µg |
| Purity | Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8 |
| Lysate Clearance Methodology | Magnetic beads or Centrifugation |
| Downstream Applications | Transfection, routine screening, restriction enzyme digestion, transformation, PCR, DNA sequencing, in vitro transcription, and other sensitive applications requiring pure DNA |
| SKU No | M1261-00 | M1261-01 | Available Separately |
|---|---|---|---|
| Purifications | 1 x 96 | 4 x 96 | |
| Solution I | 30 mL | 120 mL | Solution I |
| Solution II | 30 mL | 120 mL | Solution II |
| N3 Buffer | 40 mL | 85 mL | |
| IRD Buffer | 50 mL | 200 mL | IRD Buffer |
| VHB Buffer | 66 mL | 264 mL | VHB Buffer |
| Endo-free Water | 15 mL | 60 mL | |
| Mag-Bind® Particles RQ | 2.2 mL | 8.8 mL | Mag-Bind® Particles RQ |
| RNase A | 100 μL | 400 μL | RNase A |
| Table 1. pGEM plasmid DNA was purified from 1.5 mL DH5α cultures (n=3) using the Mag-Bind® Endo-free Plasmid Mini Kit, as well as Company P’s Kit, following manufacturers’ recommended protocol. Purified Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system. Plasmid preparation was not only endotoxin-free (<0.1 EU/μg) for Omega Bio-tek’s Kit, but the yield was also significantly higher compared to Company P’s Kit. |
Figure 1. pGEM plasmid was prepared from 1.5 mL DH5α cultures (n=3) and purified using the Mag-Bind® Endo-free Plasmid Mini Kit, as well as Company P’s equivalent Kit. Purified Plasmids were transfected into 10,000 cells with 200 ng of purified plasmid using 2 μL Lipofectamine Transfection Reagent. Plasmids purified using Omega Bio-tek’s Kit resulted in higher bioluminscent signal, indicating higher transfection efficiency compared to plasmids purified using Company P’s Kit.
Figure 2. pGEM plasmid was purified from 1 mL DH5α cultures using the Mag-Bind® Endo-free Mini Kit, fully automated on the Hamilton Microlab® STAR™ platform, starting from a bacterial pellet and using lysate clearance beads. Purified plasmid DNA was eluted in 100 μL and quantified using Thermo Scientific’s NanoDrop™ 2000c system. Plasmid yields were consistent among the replicates and had excellent purity ratios with A260/A280 ~1.8 and A260/A230 ~2.0.
Figure 3. Mag-Bind® Endo-free Plasmid Mini Kit is capable of handling diverse sample input conditions ranging from plasmid types, plasmid sizes, culture medium, and input culture volumes. The plasmid yield may vary based on these conditions.
