Automated Plasmid Purifications using the MagBinder® Fit24: Mini, Midi, and More
Purify plasmid DNA using the MagBinder Fit24.
Using the Mag-Bind® Endo-free Plasmid Mini Kit
on the MagBinder® Fit24
The Mag-Bind® Endo-free Plasmid Mini Kit (M1261) extracts high-yielding, high-quality plasmid DNA from a wide range of plasmid types and culture media:
- Transfection-quality – Endotoxin-free plasmid DNA with endotoxins levels < 0.1 EU/µg.
- Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8.
Automated Protocol Specifications
| Feature | Specification |
|---|---|
| Input Amount | up to 1.5 mL LB or TB Culture |
| Throughput | 1-24 Samples |
| Estimated Offline Processing Time | 25 minutes |
| Online Processing Time | 54 minutes |
Contact us to learn how to get this ready-to-run protocol and script:
Mag-Bind® Endo-free Plasmid Mini Kit
Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 1.5 mL culture volume.
- Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
- High-yielding – Up to 20 μg plasmid DNA from 1.5 mL TB culture
- Versatile – Process up to 1.5 mL of different culture medias with different plasmid
- Transfection-quality – Endotoxin-free plasmid DNA with endotoxins levels < 0.1 EU/µg
- Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8
Learn more about the kit:
Automated Solution Data
~1.8X higher plasmid yields and lower endotoxin levels
| Table 1. pGEM plasmid DNA was purified from 1.5 mL DH5α cultures (n=3) using the Mag-Bind® Endo-free Plasmid Mini Kit automated on the MagBinder® Fit24, as well as Company P’s Kit, following manufacturers’ recommended protocol. Purified Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system. Plasmid preparation was not only endotoxin-free (<0.1 EU/μg) for Omega Bio-tek’s Kit, but the yield was also significantly higher (~1.8X) compared to Company P’s Kit. |
Higher transfection efficiency vs Company P
Figure 1. pGEM plasmid was prepared from 1.5 mL DH5α cultures (n=3) and purified using the Mag-Bind® Endo-free Plasmid Mini Kit automated on the MagBinder® Fit24, as well as Company P’s equivalent Kit. Purified Plasmids were transfected into 10,000 cells with 200 ng of purified plasmid using 2 μL Lipofectamine Transfection Reagent. Plasmids purified using Omega Bio-tek’s Kit resulted in higher bioluminscent signal, indicating higher transfection efficiency compared to plasmids purified using Company P’s Kit.
Versatility Across Various Culture Media and Plasmid Types
Figure 2. Mag-Bind® Endo-free Plasmid Mini Kit on MagBinder® Fit24 is capable of handling diverse sample input conditions ranging from plasmid types, plasmid sizes, culture medium, and input culture volumes. The plasmid yields may vary based on these conditions.