Using the Mag-Bind® Endo-free Plasmid Mini Kit
on the Thermo Fisher Scientific KingFisher™ or compatible magnetic processor
The Mag-Bind® Endo-free Plasmid Mini Kit (M1261) extracts high-yielding, high-quality plasmid DNA from a wide range of plasmid types and culture media:
- Transfection-quality – Endotoxin-free plasmid DNA with endotoxins levels < 0.1 EU/µg.
- Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8.
Omega Bio-tek has pre-written scripts available for our Mag-Bind® line of products on the following magnetic processors:
- Accuris IsoPURE™ 96
- Accuris IsoPURE™ Mini
- Thermo Fisher Scientific KingFisher® Apex
- Thermo Fisher Scientific KingFisher® Duo Prime
- Thermo Fisher Scientific KingFisher® Flex
Due to the different customization options and variations of each instrument, please contact us for more information.
Contact us to learn how to get this ready-to-run protocol and script:
Mag-Bind® Endo-free Plasmid Mini Kit
Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 1.5 mL culture volume.
- Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
- High-yielding – Up to 20 μg plasmid DNA from 1.5 mL TB culture
- Versatile – Process up to 1.5 mL of different culture medias with different plasmid
- Transfection-quality – Endotoxin-free plasmid DNA with endotoxins levels < 0.1 EU/µg
- Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8
Automated Solution Data
Consistent Yields of High-Quality, Endotoxin-free pGEM Plasmid between Manual and Automated Methods
Figure 1. pGEM plasmid was purified from 1.5 mL LB culture volume using the Mag-Bind® Endo-free Plasmid Mini Kit on the KingFisher™ Flex. Purified plasmid was quantified using Thermo Scientific’s NanoDrop 2000c system. Plasmid yields were consistent between extraction methods, with A260/A280 ratios ~1.8, and A260/A230 ratios ~2.0. All purified plasmids were endotoxin-free (<0.01 EU/µg).