Mag-Bind® FFPE DNA Kit

Mag-Bind® FFPE DNA 96 Kit is designed for the isolation of DNA from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample. The protocol utilizes a specially formulated buffer system that not only partially reverses the formaldehyde-induced crosslinking but also ensures inhibitor-free purification of DNA. Magnetic bead-based extraction makes it suitable for both manual as well as automated processing on most open-ended liquid handling platforms as well as magnetic processors.

The Mag-Bind® FFPE DNA 96 Kit integrates a unique buffer system with the highly efficient binding properties of Mag-Bind® technology to isolate total DNA. The protocol utilizes non-toxic mineral oil in combination with heat for efficient deparaffinization of the FFPE sample eliminating the use of hazardous xylene.

Samples are first lysed in FDR Buffer aided by the presence of Proteinase K enzyme. The lysate is then heated to denature the proteinase and reverse the chemical crosslinking of the nucleic acids. Post-heating, the lysate is mixed with MB4 Buffer and Mag-Bind® Particles CH to bind DNA to the particles. Post several wash steps, DNA is eluted in Elution Buffer. Purified DNA is suitable for a variety of downstream applications including PCR, qPCR, next generation sequencing, and more.