Mag-Bind® Blood & Tissue DNA HDQ 96 Kit

$0.00$5,173.20

High-throughput DNA isolation from blood, buccal swabs, saliva & tissue using magnetic beads

  • DNA Extraction from Blood, Saliva, Buffy Coat, Buccal Swabs, Cultured Cells, and Tissues
  • Automation Friendly Formats allows for up to 384 samples to be processed in 2.5 hours
  • High Quality DNA is ready for NGS, Microarrays, and qPCR

CE-IVD marked kit is available: Mag-Bind® Blood & Tissue DNA HDQ 96 Kit CE IVD – (omegabiotek.com)

M6399-00S Mag-Bind® Blood & Tissue DNA HDQ 96 Kit (Sample)

$0.00

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

The Mag-Bind® Blood & Tissue DNA HDQ 96 Kit is designed for the rapid and reliable isolation of high-quality genomic DNA from 100-250 μL of blood samples, 500 μL saliva, swabs, mouse tails, dried blood spots, tissues, or 5 x 106 cultured cells. Mag-Bind® Particles HDQ provide quick magnetic response times, thereby reducing overall processing time.

Our cutting-edge Mag-Bind® technology reduces overall processing time by providing a quick magnetic response. This kit combines the reversible nucleic acid-binding properties of Mag-Bind® paramagnetic particles with the proven efficiency of Omega Bio-tek’s blood and tissue DNA isolation system to provide a rapid and robust method for the isolation of DNA from a variety of biological samples. The system yields high-quality DNA that is suitable for direct use in most downstream applications such as amplification, NGS, and enzymatic reactions.

Ready-to-implement protocols are available for the following platforms:

  • Accuris IsoPURE™ 96
  • Hamilton Microlab® STAR
  • Hamilton Microlab® NIMBUS
  • Tecan Fluent®
  • Tecan Freedom EVO®
  • Thermo Fisher Scientific KingFisher® Apex
  • Thermo Fisher Scientific KingFisher® Flex

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting Amount100-250 μL blood samples, saliva, swabs, mouse tails, dried blood spots or 5 x 106 cultured cells.
Elution Volume50-200 µL
TechnologyMagnetic Beads
Processing ModeAutomated, Manual
Throughput8 – 384
NoteDownstream applications: NGS, qPCR, microarray
ITEMAVAILABLE SEPARATELY
Mag-Bind® Particles HDQCall for Pricing
AL BufferView Product
TL BufferView Product
HDQ Binding BufferView Product
VHB BufferView Product
SPM BufferView Product
Proteinase K SolutionView Product
Elution BufferView Product

Yield from Whole Blood

Figure 1.  DNA yield from 200 µL whole blood extracted using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit on the ABI MagMAX 96 instrument. DNA yield was determined by PicoGreen® quantification.

Yield from Buccal Swabs

Figure 2.  Genomic DNA was extracted from buccal swab samples using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit and Company A’s genomic DNA extraction kit. 10% of DNA extracted from each sample was analyzed on a 0.8% agarose gel.

High Quality, Inhibitor-free

Figure 3.  Genomic DNA was extracted from 1,500 µL whole blood using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit. The elution volume was 500 µL. DNA was analyzed on Thermo Scientific’s NanoDrop® 2000c. 50 ng of DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors.

DNA Size

Figure 4.  The sizes of genomic DNA purified using Mag-Bind Blood & Tissue DNA HDQ Kit were determined by electrophoresis.  Sample 1 and 2 were gDNA from human blood.  L is 50 kb genomic ladder for Agilent TapeStation 2200.  The size of gDNA is around 60 kb.

Can the HDQ Binding Buffer and the Mag-Bind Particles HDQ be made into a master mix?

Yes, they can be made into a master mix before starting the experiment.

Do you have a protocol for dried blood spots using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit?

  1. Cut or punch-out the blood spot from the filter paper (up to 200 µL blood can be used per spot). Tear or cut the filter paper into small pieces and place them into a 2 mL nuclease-free microcentrifuge tube.
  2. Add 250 µL PBS.
  3. Incubate at 65 °C for 1 hour. Vortex briefly every 20 minutes.
  4. Add 25 µL Proteinase K Solution. Vortex at maximum speed for 15 seconds.
  5. Incubate at 65 °C for 30 minutes. Vortex briefly several times during incubation.
  6. Centrifuge at ≥13,000 x g for 5 minutes.
  7. Transfer the supernatant to a nuclease-free 2 mL microcentrifuge tube.
  8. Add 290 µL AL Buffer and vortex, or pipet up and down 20 times, to mix. Optional: Add 5 µL RNase A to each sample. Vortex to mix. Let sit at room temperature for 2 minutes.
  9. Continue from step 5 (page 6) of the ‘Blood Protocol’ (250 µL) on the Mag-Bind Blood & Tissue DNA HDQ 96 Kit manual.

Can the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit be used for extraction of blood in PAXgene™ tubes?

Yes. See the protocol modification below. Please be aware we have not validated this protocol.

  1. Pour all the blood from one PAXgene blood DNA tube into a 50 mL centrifuge containing 25 ml NL buffer. Mix by inverting the tube 5 – 10 times.
  2. Centrifuge for 5 minutes at 2500 x g in a swing bucket rotor. Carefully discard the supernatant.
  3. Suspend the pellet in 1 mL of PBS or elution buffer.
  4. Follow the M6399 ‘Blood Protocol’ – either scale up the protocol 4 times or run 4 parallel preps of 250 µL each.

I accidentally left the magnetic beads out when it says to store them in the fridge. Are they ok to use?

The Mag-Bind particles are stable at room temperature for 2 weeks.

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