Price range: $0.00 through $345.60
Isolate ultra-high molecular weight DNA up to 450 kb for long-read sequencing applications using magnetic beads.
All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.
Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.
Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.
Scripts available for all Mag-Bind® Kits for most open liquid handlers and common magnetic processors including the MagBinder® Fit24. Visit Automated Solutions for more information.
The Mag-Bind® HMW DNA Kit is designed for the purification of high molecular weight DNA up to 450 kb, ideal for long-read sequencing technologies. The Kit follows magnetic bead-based technology to purify high molecular weight DNA from up to 250 μL whole blood and saliva, 300 μL buffy coat, and 5 x 106 cells. The Mag-Bind® HMW DNA Kit utilizes magnetic bead -based technology and can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors.
The optimized protocol uses innovative LNB Buffer combining the lysis-bind steps into a single step. Sample is added to LNB Buffer master mixed with Mag -Bind® Particles HDQ and Proteinase K Solution for a convenient, walkaway solution. The samples are gently lysed, and DNA binds to the surface of Mag-Bind® Particles HDQ. DNA-bound to Mag-Bind® Particles HDQ undergo several wash steps to eliminate contaminants and PCR inhibitors. The protocol steps are optimized to minimize fragmentation, and high molecular weight DNA is eluted in Elution Buffer. Purified DNA is suitable for a variety of downstream applications including long-read sequencing on third-generation sequencing platforms such as Oxford Nanopore etc.
For Research Use Only. Not for use in diagnostic procedures.
| Feature | Specifications |
|---|---|
| Starting Amount | Up to 250 µL blood, 300 µL buffy coat, 5 x 106 cells, 250 µL saliva |
| Starting Material | Cultured cells, whole blood, buffy coat, saliva |
| Elution Volume | 150 µL |
| Technology | Magnetic beads |
| Processing Mode | Automated, Manual |
| Throughput | Up to 24 |
| Downstream Applications | Long-read sequencing, qPCR, Femto pulse systems etc. |
| Component | Available Separately |
|---|---|
| LNB Buffer | |
| VHB Buffer | VHB Buffer |
| BL Buffer | |
| Elution Buffer | Elution Buffer |
| Proteinase K Solution | Proteinase K |
| Mag-Bind® Particles HDQ | Mag-Bind® Particles HDQ |
| Name | Document | Languages | Link |
|---|---|---|---|
| Protocol Manual | Product Manual | English |
| Omega Bio-tek on MagBinder | Omega Bio-tek on Hamilton Microlab® STAR™ | Company N (Manual) | Company Z (Manual) | Company Q (Manual) | |
|---|---|---|---|---|---|
| Touchpoints | 8* + 4 | 7** + 1 | ~89 | ~77 | ~71 |
| DNA Binding Technology | Magnetic Beads | Large Glass Beads | Magnetic Beads | Magnetic Beads | |
| Validated Protocols for Automation | Provided by Omega Bio-tek | Cannot be Automated | Not Available | Not Available | |
Table 1. Comparison of the number of touchpoints in the HMW DNA extraction workflows for kits from Omega Bio-tek, Company N, Company Z, and Company Q, as well as DNA binding technology used and availability of validated automation protocols. Of the Kits, Omega Bio-tek’s Mag-Bind® HMW DNA Kit minimizes the number of touchpoints, while also providing validated automation protocols.
*Offline Steps – Fill buffers in the cartridge, load on to MagBinder® Fit24, add sample to pre-filled cartridge + tip mix and incubate post-elution.
**Offline Steps – Load sample, load buffers onto deck. These steps are not per sample; buffers are loaded once per run.
Figure 1. Femto pulse traces for DNA extracted using Omega Bio-tek’s kit, as well as other competitor’s kits, following manufacturer’s recommended protocols. Proportion of DNA fragments ≥ 50 kb, as depicted by Genomic Quality Number (GQN 50 kb) and average smear size in kbp based on the Femto traces is shown in the bar graph above. The Femto pulse traces, average GWN, and smear size indicate higher proportion of high-molecular-weight DNA with Omega Bio-tek’s and Company N’s kits compared to kits from Company Z and Q.
Figure 2. Genomic DNA was extracted from 250 µL blood using the Mag-Bind® HMW DNA Kit, as well as other competing kits. Yield and quality were analyzed via Thermo Scientific’s NanoDrop® 2000c. The results indicate high-yielding DNA with excellent A260/A280 and A260/A230 ratios following Omega Bio-tek’s protocol. Omega Bio-tek and Company N were comparable in terms of yield, with Company Z and Company Q resulting in lower yields. Both Company N and Company Q exhibited lower A260/A230 ratios, indicating contaminant carryover in the eluate.
| Omega Bio-tek | Company N | Company Q | |
|---|---|---|---|
| Mean Length (bp) | 20444 | 23181 | 13808 |
| Median Quality | 21.1 | 22.5 | 21.3 |
| % Reads > 50 kb | 14.4 | 15.3 | 4.1 |
| % Reads > 100 kb | 3.8 | 2.0 | 0.1 |
| Longest Read (bp) | 448K | 958K | 205K |
Figure 3. Genomic DNA was extracted from 250 µL whole blood using the Mag-Bind® HMW DNA Kit, as well as other competing kits, was sequenced on Oxford Nanopore Platform for ultra-long reads. Sequencing summary data comparing different extraction methods is shown above. All metrics indicate that Omega Bio-tek’s chemistry extracts high-molecular-weight DNA fragments with the longest read around 450 kb and % reads >50 kb and >100 kb was highest compared to competing chemistries. The N50 metric, which quantifies the read length above which half of the data is derived, was also found to be greater for Omega Bio-tek compared to Company N and Company Q.
Figure 4. Genomic DNA was extracted from 250 µL whole blood, 5 x 106 cells, 100 µL buffy coat, and 250 µL saliva using the Mag-Bind® HMW DNA Kit both manually and using the MagBinder® Fit24 Nucleic Acid Purification System. Yield and purity were analyzed via NanoDrop® 2000c, and size distribution was analyzed via pulsed-field gel electrophoresis. DNA extracted from saliva is expected to exhibit increased fragmentation due to enzymatic degradation and mechanical stress associated with the collection and storage of saliva samples.
Figure 4. Genomic DNA was extracted from 250 µL whole blood derived from four independent lots following manual and automated extraction on the Hamilton Microlab® STAR™ platform. Among all lots, yield values were comparable between manual and automated extraction methods. Size distribution of high-molecular-weight fragments was visualized using pulsed-field gel electrophoresis (PFGE). Every extraction resulted in high-quality DNA with A260/A280 ratios ~1.8 and A260/A230 ratios ~2.2.
Figure 5. Genomic DNA was extracted from 250 µL whole blood derived from four independent lots following manual and automated extraction on the Hamilton Microlab® STAR™ platform and subjected to qPCR amplification. The average Ct values differed by ~3.3 cycles per 10-fold dilution, indicating inhibitor-free DNA extracted using Omega Bio-tek’s Kit.
Figure 6. Genomic DNA was extracted by 3 independent users from 250 µL whole blood following manual and automated extraction on the MagBinder® Fit24 Nucleic Acid Purification System. Yields and purity ratios were quantified via Thermo Scientific’s NanoDrop™ 2000c. The size distribution of high-molecular-weight fragments was visualized using pulsed-field gel electrophoresis (PFGE). Yields were comparable between manual and automated methods among each independent user test. PFGE analysis validates extraction of HMW DNA >242.5 kb.
Figure 7. Genomic DNA was extracted by 3 independent users from 250 µL whole blood following manual and automated extraction on the MagBinder® Fit24 Nucleic Acid Purification System. and subjected to qPCR amplification. The average Ct values differed by ~3.3 cycles per 10-fold dilution, indicating inhibitor-free DNA extracted using Omega Bio-tek’s Kit.
| Name | Document | Languages | Link |
|---|---|---|---|
| Protocol Manual | Product Manual | English |
| Name | Document | Languages | Link |
|---|---|---|---|
| Protocol Manual | Product Manual | English |
| Components | Hazard Standards | Languages | Link | hf:tax:dlp_document_language | hf:tax:dlp_document_hazard-standard |
|---|---|---|---|---|---|
| BL Buffer | GHS | English | english | ghs | |
| BL Buffer | GHS | Spanish | spanish | ghs | |
| BL Buffer | REACH | English | english | reach | |
| BL Buffer | REACH | Danish | danish | reach | |
| BL Buffer | REACH | Finnish | finnish | reach | |
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| BL Buffer | WHMS | English | english | whms | |
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| BL Buffer | REACH | Latvian | latvian | reach | |
| BL Buffer | REACH | Polish | polish | reach | |
| BL Buffer | REACH | Portuguese | portuguese | reach | |
| BL Buffer | REACH | Greek | greek | reach | |
| Elution Buffer | GHS | English | english | ghs | |
| Elution Buffer | GHS | Spanish | spanish | ghs | |
| Elution Buffer | REACH | English | english | reach | |
| Elution Buffer | REACH | Danish | danish | reach | |
| Elution Buffer | REACH | Finnish | finnish | reach | |
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| Elution Buffer | WHMS | English | english | whms | |
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| LNB Buffer | GHS | English | english | ghs | |
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| LNB Buffer | WHMS | English | english | whms | |
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| LNB Buffer | REACH | Spanish | spanish | reach | |
| Mag-Bind Particles HDQ | GHS | English | english | ghs | |
| Mag-Bind Particles HDQ | REACH | English | english | reach | |
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| Mag-Bind Particles HDQ | WHMS | English | english | whms | |
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| Mag-Bind Particles HDQ | GHS | Spanish | spanish | ghs | |
| Proteinase K Solution | GHS | English | english | ghs | |
| Proteinase K Solution | GHS | Spanish | spanish | ghs | |
| Proteinase K Solution | REACH | English | english | reach | |
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| VHB Buffer | GHS | English | english | ghs | |
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