Mag-Bind® NGS Normalization Kit
Price range: $0.00 through $794.90
High-throughput normalization for sequencing-ready libraries.
- Reliable Library Normalization – Achieve consistent normalization and get sequence-ready
- Quantification-Free Library Pooling – Skip the quants and dilutions for automated, sequence-ready libraries.
- Maximize Throughput – Fully automated using leading open ended liquid handling platforms (e.g. Hamilton®, Dynamic Devices Lynx)
- qPCR-level Accuracy – Ensure normalization without qPCR complexity
The Mag-Bind® NGS Normalization Kit is designed for normalizing libraries for use in Next Generation Sequencing workflows. The Kit follows magnetic bead-based methodology to facilitate normalization of libraries or PCR amplicons starting with at least 200 ng input and resulting in ~100 ng normalized output. The Kit eliminates manual library quantification and dilution, reducing pipetting steps, time, and tip costs.
The Mag-Bind® NGS Normalization Kit follows an easy and fast workflow and is compatible with most open -ended automation platforms. The protocol follows a simple bind -wash-elute routine. Briefly, the libraries at different concentrations are bound to
Mag-Bind® Particles DDB in the presence of a binding buffer. The magnetic beads have a limited binding capacity allowing only a predetermined amount of DNA to be captured and eluted to a fixed concentration. The output concentration can be adjusted by adjusting the elution volume used. Once normalized, the different libraries can be pooled at equal volumes so that they are evenly represented in downstream sequencing workflows.
For Research Use Only. Not for use in diagnostic procedures.
| Feature | Specifications |
|---|---|
| Starting Amount | 200 ng to 2000 ng |
| Starting Material | NGS libraries, PCR amplicons |
| Elution Volume | 25 µL |
| Technology | Magnetic beads |
| Processing Mode | Automated, Manual |
| Throughput | 1 - 96 |
| Downstream Applications | Next Generation Sequencing Workflows |
| Component | Available Separately |
|---|---|
| CB Buffer | |
| Elution Buffer | Elution Buffer |
| Mag-Bind® Particles DDB |
Product Literature
Target Coverage by Normalization Method
Figure 1. Target coverage was determined from 6 targeted DNA libraries with 3 different inputs from different normalization methods. The Mag-Bind NGS Normalization kit yields coverage equivalent to that of qPCR-based and Qubit-based normalization methods. All normalization methods resulted in high quality sequencing depth and even coverage across targeted regions.
Normalization of Targeted DNA Libraries
Figure 2. Targeted DNA library preparation from six libraries from a diverse input range was normalized by different methods. All normalization methodologies resulted in uniform library representation irrespective of the input amount.
Sequencing Quality Score (Q30)
Figure 3. Omega Bio-tek’s Mag-Bind® NGS Normalization kit resulted in at least 92% of sequenced bases that have a Phred quality score (Q-score) of 30 or higher similar to qPCR- and Qubit-based normalization methods. High quality scores provide reliable sequencing data with minimal misalignments and erroneous variants.
Multilength Library Normalization
Figure 4. DNA return was measured across various DNA input amounts for 300 bp (A), 500 bp (B), and 700 bp (C) library lengths. For each library length tested, normalization can be seen from 200 ng input to 2000 ng input.