$0.00 – $2,770.40
All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.
Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.
Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.
The Mag-Bind® PX Blood RNA 96 Kit provides a convenient and fast RNA extraction solution for samples stabilized in PreAnalytiX PAXgene™ blood RNA tubes. This system combines Mag-Bind® particles with HiBind® silica technologies for purification of total RNA from up to 2.5 mL preserved blood samples.
The blood sample is spun down and the crude RNA/DNA pellet is collected and washed. The pellet is then resuspended and digested with Proteinase K. The lysate is applied to a filter plate to remove genomic DNA. This procedure completely removes inhibitors and sample stabilization reagents to allow for reliable downstream analysis. High quality purified RNA can be used for downstream applications such as qRT-PCR, RT-PCR, and microarray analysis.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification |
| Elution Volume | 50-100 µL |
| Starting Material | Blood in PAXgene RNA tube |
| Starting Amount | Up to 2.5 mL |
| RNA Yield | Scalable |
| Processing Mode | Automated; Manual |
| Throughput | 96 Samples per run |
| RNA Binding Technology | Magnetic Beads |
| Binding Capacity | Scalable |
Figure 1. RNA Integrity Assessment. The quality of the RNA purified was assessed by the RNA Integrity Score (RINe) determined from the electrophoretic trace and Agilent’s TapeStation® 2200 system.
Figure 2. Electropherogram Representing Purified RNA. 2.5 mL whole blood was drawn to PAXgene™ blood RNA tubes and stored at room temperature for 24 hours. RNA was purified following manufacturer’s recommended protocols. RNA was analyzed on Agilent’s TapeStation® 2200.
