Price range: $0.00 through $1,156.50
Compatible with industry leading preservation media such as Omega Bio-tek’s RNA-Lock Reagent, Zymo’s DNA/RNA Shield or Thermo Fisher’s RNAlater.
All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.
Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.
Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.
Scripts available for all Mag-Bind® Kits for most open liquid handlers and common magnetic processors including the MagBinder® Fit24. Visit Automated Solutions for more information.
The Mag-Bind® Total RNA Xpress Kit allows for rapid and reliable isolation of high-quality total RNA including small RNAs from a wide variety of tissue and cultured cells. Total RNA can be purified from up to 25 mg tissue or 1 x 106 cultured cells. The Kit follows most magnetic bead-based technology and can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors.
If using the Mag -Bind® Total RNA Xpress Kit for the first time, please read this manual in its entirety to become familiar with the procedures. Samples are first lysed in OTRK Lysis Buffer supplemented with DTT. The lysate is cleared using centrifugation and
total nucleic acids are then bound to Mag -Bind® Particles RQ in the presence of isopropanol. Genomic DNA bound to the beads is eliminated through a DNase I digestion step while preserving the integrity of the RNA bound to the beads. The beads are subjected to two quick alcohol washes to remove contaminants. High-quality RNA is then eluted in Nuclease-free Water and is ready for use in a wide range of downstream applications such as RT-PCR, RT-qPCR, microarray analysis, next-generation RNA sequencing (RNA-Seq), etc.
For Research Use Only. Not for use in diagnostic procedures.
| Feature | Specifications |
|---|---|
| Starting Amount | Up to 25 mg tissue, 106 cells |
| Starting Material | Cultured cells, tissue |
| Elution Volume | 50 - 100 µL |
| Technology | Magnetic beads |
| Processing Mode | Automated, Manual |
| Throughput | 1 - 96 |
| Downstream Applications | RT-PCR, RT-qPCR, microarray analysis, next -generation RNA sequencing (RNA -Seq), etc.ensitive applications requiring pure DNA |
| Component | Available Separately |
|---|---|
| OTRK Lysis Buffer | |
| VHB Buffer | VHB Buffer |
| Nuclease-free Water | |
| Mag-Bind® DNase I | |
| NR1 Buffer | |
| Proteinase K Solution | Proteinase K |
| Mag-Bind® Particles RQ | Mag-Bind® Particles RQ |
Figure 2. Average RNA yields from 10 mg of different tissue types and one million AD293 cells using Omega Bio-tek’s Mag-Bind® Total RNA Xpress Kit and comparable kits from Company T and Company Q following manufacturer’s recommended protocols. Yields were measured by UV spectrophotometry. Total RNA yield using Omega Bio-tek’s kit was superior for all the samples tested except for brain (Company Q) and muscle (Company T).
Figure 3. Average RNA absorbance ratios from 10 mg of different tissue types and one million AD293 cells using Omega Bio-tek’s Mag-Bind® Total RNA Xpress Kit and comparable kits from Company T and Company Q following manufacturer’s recommended protocols. Purity of RNA isolated using different manufacturers’ kits analyzed through spectrophotometry focusing on A260/A280 and A260/A230 ratios.
Figure 4. Average Ct values obtained on 10X and 100X dilutions of RNA purified from 10 mg of different tissue types and one million AD293 cells using Omega Bio-tek’s Mag-Bind® Total RNA Xpress Kit. The Ct values differed by ~ 3.3 cycles per 10-fold dilution indicating inhibitor-free RNA extracted from all the samples extracted using Omega Bio-tek’s kit.
Figure 5. TapeStation® analysis of RNA purified from 10 mg liver using Omega Bio-tek’s Mag-Bind® Total RNA Xpress Kit and a comparable kit from Company Q. Omega Bio-tek’s kit was capable of purifying total RNA which includes small RNAs; small RNAs were missing in the total RNA purified using Company Q’s kit.
Figure 6. DNA and RNA was extracted from 10 mg murine liver tissue preserved in either Omega Bio-tek’s RNA-Lock Reagent, Zymo’s DNA/RNA Shield or Thermo Fisher’s RNAlater. DNA and RNA was eluted in 100 µL. The results corroborate the compatibility of Mag-Bind® Total RNA Xpress Kit with various tissue storage and preservation solutions on the market.
| Average Ct Values | ||||
|---|---|---|---|---|
| Omega Bio-tek | Company T | Company Q | ||
| Mouse Liver | Undetermined | 21.3 | 29.6 | |
| Mouse Muscle | Undetermined | 24.5 | 32.3 | |
| Mouse Brain | Undetermined | 23.8 | 26.9 | |
| AD293 Cells | Undetermined | 33.8 | 33.2 | |
| Murine DNA Positive Control | 18.7 | |||
| Human DNA Positive Control | 22.2 | |||
| NTC | Undetermined | |||
Table 1. Average Ct values obtained from RNA purified from 10 mg of different tissue types and one million AD293 cells using the Mag-Bind® Total RNA Xpress Kit, as well as two comparable competing kits. RNA extracted from each sample type using Omega Bio-tek’s Kit exhibited no detectable DNA from qPCR, while RNA extracted using the two competing kits showed low but detectable amounts of DNA. The positive controls for murine and human DNA showed detectable amounts of DNA while the No Template Control detected no DNA.
