E.Z.N.A.® Plasmid DNA Maxi Kit
$0.00 – $1,113.00
- Speed – Purification of plasmid DNA in < 60 minutes
- Safety – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High quality – DNA is suitable for a variety of downstream applications
The E.Z.N.A.® Plasmid DNA Maxi Kit combines the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiBind® DNA columns facilitate the binding, washing, and elution steps, thus enabling multiple samples to be processed simultaneously. Up to 600-1200 µg high copy number plasmid DNA or 50-300 µg low copy number plasmid DNA can be purified from 50-200 mL overnight culture using E.Z.N.A.® Plasmid DNA Maxi Kit. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations.
The E.Z.N.A. Plasmid Maxi Kit can isolate up to 250 μg and 1.2 mg of plasmid DNA from 50 mL and 200 mL bacterial cultures, respectively. The kit uses a modified alkaline lysis method to lyse the cells and separate genomic DNA from plasmid DNA. Cellular debris are removed by centrifugation, and the protocol follows a simple bind, wash, and elute procedure to deliver high-quality plasmid DNA. The system not only eliminates the time-consuming isopropanol precipitation step required by gravity flow columns but also the need for expensive accessories.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc. |
| Starting material | Optimal volume depends on culture density and plasmid copy number. The optimal cell mass (OD600 x mL culture) is 80-100. |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing mode | Manual (centrifugation or vacuum) |
| Throughput | 1 – 24 |
| DNA binding technology | Silica Maxi Spin Column |
| Lysate clearance method | Centrifugation |
| Processing time | <60 minutes |
| Yield | 600-1200 µg for high copy-number; 50-300 µg for low copy-number |
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| HiBind® DNA Maxi Columns | View Product |
| 50 mL Collection Tubes | — |
| Solution I | View Product |
| Solution II | View Product |
| Solution III | View Product |
| HBC Buffer | View Product |
| DNA Wash Buffer | View Product |
| RNase A | View Product |
| Elution Buffer | View Product |
Figure 1. pGEM plasmid was purified from 250 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Maxi Kit following manufacturer’s recommended protocols. Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system.
