Mag-Bind® Universal Pathogen 96 Kit

on the MagBinder® Fit24

High-throughput DNA & viral RNA isolation from a variety of sample sources

  • Ceramic Beads Pre-Aliquoted to 96-well Plates
  • Isolates Yeast, Fungal, Bacterial, and Viral DNA
  • Isolate Viral RNA

Automated Protocol Specifications

Feature Specification
Input Amount 250 μL Serum, Stool, or Urine
Throughput 1-24 Samples
Estimated Offline Processing Time 45 minutes
Online Processing Time 27 minutes

Contact us to learn how to get this ready-to-run protocol and script:

M4029 on a MagBinder Information Request
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Mag-Bind® Universal Pathogen 96 Kit

High-throughput DNA & viral RNA isolation from a variety of sample sources

  • Ceramic Beads Pre-Aliquoted to 96-well Plates
  • Isolates Yeast, Fungal, Bacterial, and Viral DNA
  • Isolate Viral RNA

Available with or without disrupter plates!

Learn more about the kit:

Mag-Bind® Universal Pathogen 96 Kit

 

Automated Solution Data

Inhibitor-free Isolation of Gram Positive Bacterial DNA from Plasma

Figure 1. 100 µL Influenza A/B and 50 µL of Bacillus subtilis were spiked into 100 µL plasma. DNA was isolated using the Mag-Bind® Universal Pathogen 96 Kit on the MagBinder® Fit24 and compared to manual extraction methods. qPCR targeting B. sub was performed on 10-fold and 100-fold dilutions. The ΔCt between dilutions was comparable between manual and automated isolation methods, ~3.3, indicating inhibitor-free isolation.

Inhibitor-free Isolation of Gram-Positive Bacterial DNA from Urine Using the MagBinder® Fit24

Figure 2. 50 µL of Bacillus subtilis was spiked into 200 µL urine. DNA was isolated using the Mag-Bind® Universal Pathogen 96 Kit on the MagBinder® Fit24 and compared to manual extraction methods. qPCR targeting B. sub was performed on 10-fold and 100-fold dilutions. The ΔCt between dilutions was comparable between manual and automated isolation methods, ~3.3, indicating inhibitor-free isolation.

Influenza Detection from Cells using the MagBinder® Fit24

Figure 3. 75 µL of Influenza A/B was spiked into 175 µL cells. DNA was isolated using the Mag-Bind® Universal Pathogen 96 Kit on the MagBinder® Fit<sup>24</sup> and compared to manual extraction methods. qPCR targeting Influenza was performed on 2 µL and 4 µL dilutions. The ΔCt between dilutions was comparable between manual and automated isolation methods.

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