Automated Plasmid Purifications using the MagBinder® Fit24: Mini, Midi, and More
Purify plasmid DNA using the MagBinder Fit24.
Using the Mag-Bind® Endo-free Plasmid Midi Kit
on the MagBinder® Fit24
The Mag-Bind® Endo-free Plasmid Midi Kit (M1272) extracts high-yielding, high-quality plasmid DNA from a wide range of plasmid types and culture media:
- High-yielding – Plasmid DNA yield up to 200 μg
- Versatile – Process up to 50 mL bacterial culture with different plasmid types and culture medias
- Transfection-quality – Endotoxin free plasmid DNA with endotoxins levels < 0.1 EU/µg
- Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8
Automated Protocol Specifications
| Feature | Specification |
|---|---|
| Input Amount | up to 50 mL LB or 10 TB Culture |
| Throughput | 1-24 Samples |
| Estimated Offline Processing Time | 40 minutes |
| Online Processing Time | 64 minutes |
Contact us to learn how to get this ready-to-run protocol and script:
Mag-Bind® Endo-free Plasmid Midi Kit
Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 50 mL culture volume.
- Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
- High-yielding – Plasmid DNA yield up to 200 μg
- Rapid – 24 samples can be processed in ~ 70 minutes starting from a bacterial pellet without manual intervention
- Versatile – Process up to 50 mL bacterial culture with different plasmid types and culture medias
- Transfection-quality – Endotoxin free plasmid DNA with endotoxins levels < 0.1 EU/µg
- Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8
Learn more about the kit:
Mag-Bind® Endo-free Plasmid Midi Kit
Automated Solution Data
Significantly higher Plasmid yields compared to Company T
Figure 1. pGEM and pGL4.51 plasmids were purified from 5 mL DH5α cultures (n=3) using the Mag-Bind® Endo-free Plasmid Midi Kit on the MagBinder® Fit24 Nucleic Acid Purification System, as well as Company T’s Kit following manufacturers’ recommended centrigation protocols. Purified Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system. Plasmid purification was not only endotoxin-free (<0.1 EU/μg) for Omega Bio-tek’s Kit, but the yield was also significantly higher compared to Company T’s Kit.
Higher transfection efficiency vs. Company T
Figure 2. Plasmid pGL4.51 was prepared from 5 mL DH5α cultures (n=3) and purified using the Mag-Bind® Endo-free Plasmid Midi Kit on the MagBinder® Fit24 Nucleic Acid Purification System, as well as Company T’s equivalent Kit. Purified Plasmids were transfected into 10,000 cells with 200 ng of purified plasmid using 2 μL Lipofectamine Transfection Reagent. Plasmids purified using Omega Bio-tek’s Kit resulted in higher bioluminscent signal, indicating higher transfection efficiency compared to plasmids purified using Company T’s Kit.
Excellent Plasmid Yields and Purity Extracted on MagBinder® Fit24 Platform
Figure 3. pGEM plasmid was purified from 25 mL and 50 mL LB as well as 10 mL TB media (n=3) using Mag-Bind® Endo-free Plasmid Midi Kit automated on the MagBinder® Fit24 Platform following three different lysate clearance methodologies. Yields and quality were comparable, irrespective of the lysate clearance methodology used.