Mag-Bind® Universal Pathogen 96 Kit
on the Thermo Fisher Scientific KingFisher™ or compatible magnetic processor
High-throughput DNA & viral RNA isolation from a variety of sample sources
- Ceramic Beads Pre-Aliquoted to 96-well Plates
- Isolates Yeast, Fungal, Bacterial, and Viral DNA
- Isolate Viral RNA
Omega Bio-tek has pre-written scripts available for our Mag-Bind® line of products on the following magnetic processors:
- Accuris IsoPURE™ 96
- Accuris IsoPURE™ Mini
- Thermo Fisher Scientific KingFisher® Apex
- Thermo Fisher Scientific KingFisher® Duo Prime
- Thermo Fisher Scientific KingFisher® Flex
Due to the different customization options and variations of each instrument, please contact us for more information.
Contact us to learn how to get this ready-to-run protocol and script:
Mag-Bind® Universal Pathogen 96 Kit
High-throughput DNA & viral RNA isolation from a variety of sample sources
- Ceramic Beads Pre-Aliquoted to 96-well Plates
- Isolates Yeast, Fungal, Bacterial, and Viral DNA
- Isolate Viral RNA
Available with or without disrupter plates!
Mag-Bind® Universal Pathogen 96 Kit
Automated Solution Data
Inhibitor-free Gram-Positive Bacteria Isolation
Figure 1. Gram Positive Bacteria Bacillus subtilis was spiked into stool samples and isolated using the Mag-Bind® Universal Pathogen 96 Kit. Automated isolation was performed using the KingFisher Flex and compared to manual isolation methods. qPCR was performed in triplicate on 10-fold and 100-fold dilutions. The ΔCt across dilutions was ~3.3 for both manual and automated methods, indicating minimal inhibition.
Inhibitor-free Yeast Isolation
Figure 2. Yeast species Saccharomyces cerevisiae was spiked into stool samples and isolated using the Mag-Bind® Universal Pathogen 96 Kit. Automated isolation was performed using the KingFisher Flex and compared to manual isolation methods. qPCR was performed in triplicate on 10-fold and 100-fold dilutions. The ΔCt across dilutions was ~3.3 for both manual and automated methods, indicating minimal inhibition.
Consistent, Automated Gram-Negative Bacteria Isolation
Figure 3. The gram-negative bacteria E. coli was spiked into serum and urine samples and isolated using the Mag-Bind® Universal Pathogen 96 Kit. Automated isolation was performed using the KingFisher Flex. qPCR was performed in triplicate on undiluted and 10-fold dilutions. The ΔCt across dilutions was consistent for both sample types
Consistent Protozoan Isolation
Figure 4. Cryptosporidium oocysts were spiked into corresponding sample types and isolated using the Mag-Bind® Universal Pathogen 96 Kit automated on the KingFisher Flex. 20 µL SYBR® qPCR was performed in triplicate on primers specific for the target organism. Average of triplicate data shown. The ΔCt across dilutions was consistent for all sample types.
Detection of Viral DNA Across Sample Types
Figure 5. Hepatitis B virus was spiked into serum and urine samples and isolated using the Mag-Bind® Universal Pathogen 96 Kit. Automated isolation was performed using the KingFisher Flex. qPCR was performed in triplicate on undiluted and 10-fold dilution.
Detection of Viral RNA Across Sample Types
Figure 6. Influenza A/B virus was spiked into stool, urine, and serum samples and isolated using the Mag-Bind® Universal Pathogen 96 Kit. Automated isolation was performed using the KingFisher Flex. qPCR was performed in triplicate on undiluted and 10-fold dilution.