Effortlessly Isolate High-Quality DNA from Molluscs & Insects
Efficiently isolates DNA from molluscs, insects, arthropods, roundworms, flatworms, and other polysaccharide-rich tissues
Why Choose the E.Z.N.A.® Mollusc & Insect DNA Kit?
The E.Z.N.A.® Mollusc & Insect DNA Kit is the ultimate solution for researchers looking to efficiently extract genomic DNA from a wide range of invertebrate samples. From molluscs to insects including formalin preserved specimens, this kit ensures high yields with unmatched purity.
- Versatility Across Sample Types – Efficiently isolates DNA from molluscs, insects, arthropods, roundworms, flatworms, and other polysaccharide-rich tissues.
- High Purity & High Yield – Achieve A260/A280 ratios of 1.7-1.9, ensuring up to 90% DNA purity suitable for PCR, thermal cycle amplification, and hybridization.
- Streamlined Workflow – Save time with a rapid alcohol precipitation step and easy-to-use HiBind® DNA Mini Columns.
Jeffrey C Nekola, Ph.D.
Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, CZ-611 37 Brno, Czech Republic
How does the E.Z.N.A.® Mollusc & Insect DNA Kit efficiently recover DNA?
The E.Z.N.A.® Mollusc & Insect DNA Kit is designed for the efficient recovery of genomic DNA from molluscs, insects, arthropods, roundworms, flatworms, and other invertebrate tissue samples rich in mucopolysaccharides. This kit can also be used with formalin preserved material or invertebrates frozen or preserved in alcohol or DNE solution.
The procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding technology of Omega Bio-tek’s HiBind® matrix. Samples are homogenized and lysed in a high salt buffer containing CTAB and extracted with chloroform to remove mucopolysaccharides. Following a rapid alcohol precipitation step, DNA is bound to the HiBind® DNA Mini Column upon adjustment of binding conditions and further purified through multiple wash steps. In this way, salts, proteins and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.
Learn more about the kit and view the product data:
E.Z.N.A.® Mollusc & Insect DNA Kit
| FEATURES | SPECIFICATIONS |
|---|---|
| Starting Amount | 50 mg |
| Starting Material | Molluscs, insects, arthropods, roundworms, flatworms, and other invertebrate tissue samples rich in mucopolysaccharides |
| Yield | 100 µg |
| Elution Volume | 50-100 μL |
| Technology | HiBind® DNA Mini Column |
| Processing Mode | Manual |
Open Access Publications using the E.Z.N.A.® Mollusc & Insect DNA Kit
Sierra, M.A., Ryon, K.A., Tierney, B.T. et al. Microbiome and metagenomic analysis of Lake Hillier Australia reveals pigment-rich polyextremophiles and wide-ranging metabolic adaptations. Environmental Microbiome 17, 60 (2022). https://doi.org/10.1186/s40793-022-00455-9
Ge, M., Wang, D., Liang, H., Zhu, J., Shi, X., & Tian, J. (2022). The complete mitochondrial genome of Fannia canicularis (Diptera: Fanniidae). Mitochondrial DNA Part B, 7(10), 1841–1842. https://doi.org/10.1080/23802359.2022.2134744
, , , , , , Genotoxicity Evaluation of Metformin in Freshwater Planarian Dugesia japonica by the Comet Assay and RAPD Analysis, BioMed Research International, 2022, 2822605, 7 pages, 2022. https://doi.org/10.1155/2022/2822605
2020. Conserved novel ORFs in the mitochondrial genome of the ctenophore Beroe forskalii. PeerJ 8:e8356 https://doi.org/10.7717/peerj.8356