E.Z.N.A.® SP Plant DNA Kit
$0.00 – $781.60
- Rapid – Homogenizer Columns allow for faster processing
- Reproducible – Standardized method for reliable results from a variety of samples
- Safe – No organic extractions
- High-quality – Purified DNA suitable for most applications
The E.Z.N.A.® SP Plant DNA Kit is specially designed for the rapid and reliable isolation of high-quality total cellular DNA from a variety of plant species and tissues. Up to 100 mg of fresh sample or 30 mg of dry sample can be processed in less than 40 minutes. The optimized procedure incorporates the homogenizer spin column, a unique filter filtration and homogenization column that can efficiently remove cell debris and improve the sample handling following the sample lysis. Purified DNA is suitable for most downstream applications to include PCR, restriction enzyme digestion, and hybridization techniques.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Starting Amount | Up to 50 mg wet tissue |
| Starting Material | Fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content |
| Elution Volume | 50-100 μL |
| Technology | HiBind® DNA Mini Column |
| Processing Mode | Manual |
| Throughput | 1-24 |
| Note | SDS lysis,with a homogenizer column |
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| HiBind® DNA Mini Columns | View Product |
| 2 mL Collection Tubes | View Product |
| Homogenizer Mini Columns | View Product |
| SP1 Buffer | View Product |
| SP2 Buffer | View Product |
| SP3 Buffer | View Product |
| SPW Buffer | View Product |
| RNase A | View Product |
| Elution Buffer | View Product |
Successful PCR
Figure 1. DNA was isolated from 100 mg peanut leaf using Omega Bio-tke’s SP Plant DNA Kit. A serial dilution of recovered DNA was then used in SYBR® Green real-time PCR targeting a 91 bp fragment of the Alah1 gene. Each reaction was performed in triplicate. The lines represent input DNA amounts of 30, 3, 0.3, and 0.03 ng of DNA.
Yield Comparison
Figure 2. Average yields obtained from 100 mg of fresh leaf tissue from various plant samples.