E.Z.N.A.® Plant DNA DS Kit


Isolate gDNA from plant samples using spin columns.

  • Rapid – Homogenizer Columns allow for faster processing
  • Versatile – Reliable results from a variety of sample types
  • Safe – No organic extractions
  • High-quality – Purified DNA suitable for most applications

The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications.

For Research Use Only. Not for use in diagnostic procedures.

Starting AmountUp to 50 mg wet tissue
Starting MaterialFresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content
Elution Volume50-100 μL
TechnologyHiBind® DNA Mini Column
Processing ModeManual
NoteCTAB lysis
HiBind® DNA Mini Columns View Product
Homogenizer Mini Columns View Product
2 mL Collection Tubes View Product
CSPL Buffer View Product
Proteinase K Solution View Product
RBB Buffer View Product
XP2 Binding Buffer View Product
HBC Buffer View Product
DNA Wash Buffer View Product
Elution Buffer View Product
CSPL Buffer View Product
RNase A View Product

DNA Yield Comparison

Figure 1. Comparison of DNA yield from multiple crops. 40-50 mg of respective fresh leaf tissue was extracted in triplicate according to manufacturer’s recommended protocols and eluted in 100 µL. DNA analyzed with fluorescent DNA-based quantification method. Total yield was divided by total tissue amount to show ng of DNA per mg of leaf tissue.

qPCR Comparison

Figure 2. qPCR comparison from corn samples. Real-time PCR with corn-specific primers was performed on triplicates of undiluted, 10-fold and 100-fold dilutions of DNA. DNA was isolated using Omega Bio-tek’s E.Z.N.A.® Plant DNA DS Kit and a comparable column-based kit from Company Q. Omega Bio-tek’s kit not only has significantly higher yields but also less qPCR inhibition when compared to that of Company Q’s.

Yield & Quality

Figure 3. Genomic DNA was purified from either 50 mg potato leaf or 30 mg corn leaf powder with the E.Z.N.A. Plant DNA DS Kit. DNA concentration determined by optical density measurements with NanoDrop® 2000c. Total elution volume was 100 µL.

Size Analysis

Figure 4. Genomic DNA was purified from 50 mg potato leaf with the E.Z.N.A. Plant DNA DS Kit.  5 µL eluate DNA was analyzed on a 1% Agarose gel.

Ct Value Comparison

Figure 5. Genomic DNA was extracted from 50 mg potato leaf and 30 mg corn lead powder using the E.Z.N.A. Plant DNA DS Kit . 2 µL of Eluted DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. C: inhibitor-free control; T: gDNA samples. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors

Product Information

Quick GuideProduct ManualEnglish
Protocol ManualProduct ManualEnglish
E.Z.N.A.® Plant DNA DS KitProduct LiteratureEnglish
Rapid, High Performance and Cost-Effective Plant DNA ExtractionsApplication NoteEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
CSPL BufferGHSSpanishspanishghs
CSPL BufferGHSEnglishenglishghs
CSPL BufferWHMSFrenchfrenchwhms
CSPL BufferWHMSEnglishenglishwhms
CSPL BufferREACHSwedishswedishreach
CSPL BufferREACHSpanishspanishreach
CSPL BufferREACHNorwegiannorwegianreach
CSPL BufferREACHGermangermanreach
CSPL BufferREACHFrenchfrenchreach
CSPL BufferREACHFinnishfinnishreach
CSPL BufferREACHDanishdanishreach
CSPL BufferREACHEnglishenglishreach
CSPL BufferREACHItalianitalianreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferGHSEnglishenglishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHGreekgreekreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHDutchdutchreach
Elution BufferWHMSFrenchfrenchwhms
Elution BufferWHMSEnglishenglishwhms
Elution BufferREACHSwedishswedishreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHGermangermanreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHDanishdanishreach
Elution BufferGHSSpanishspanishghs
Elution BufferGHSEnglishenglishghs
HBC BufferWHMSFrenchfrenchwhms
HBC BufferWHMSEnglishenglishwhms
HBC BufferREACHSwedishswedishreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHEnglishenglishreach
HBC BufferGHSSpanishspanishghs
HBC BufferREACHGermangermanreach
HBC BufferGHSEnglishenglishghs
Proteinase K SolutionREACHGreekgreekreach
Proteinase K SolutionREACHPortugueseportuguesereach
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionGHSEnglishenglishghs
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHNorwegiannorwegianreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHFinnishfinnishreach
RBB BufferREACHEnglishenglishreach
RBB BufferGHSEnglishenglishghs
RBB BufferWHMSFrenchfrenchwhms
RBB BufferWHMSEnglishenglishwhms
RBB BufferREACHSwedishswedishreach
RBB BufferREACHSpanishspanishreach
RBB BufferREACHItalianitalianreach
RBB BufferGHSSpanishspanishghs
RBB BufferREACHDanishdanishreach
RBB BufferREACHFinnishfinnishreach
RBB BufferREACHFrenchfrenchreach
RBB BufferREACHGermangermanreach
RBB BufferREACHNorwegiannorwegianreach
XP2 Binding BufferGHSEnglishenglishghs
XP2 Binding BufferGHSSpanishspanishghs
XP2 Binding BufferREACHEnglishenglishreach
XP2 Binding BufferREACHDanishdanishreach
XP2 Binding BufferREACHFinnishfinnishreach
XP2 Binding BufferREACHFrenchfrenchreach
XP2 Binding BufferREACHGermangermanreach
XP2 Binding BufferREACHItalianitalianreach
XP2 Binding BufferREACHNorwegiannorwegianreach
XP2 Binding BufferREACHSpanishspanishreach
XP2 Binding BufferREACHSwedishswedishreach
XP2 Binding BufferWHMSEnglishenglishwhms
XP2 Binding BufferWHMSFrenchfrenchwhms
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