E.Z.N.A.® Plant DNA DS Kit


Isolation of genomic DNA from plant samples with high amounts of polysaccharides & polyphenols.

  • Rapid – Homogenizer Columns allow for faster processing
  • Versatile – Reliable results from a variety of sample types
  • Safe – No organic extractions
  • High-quality – Purified DNA suitable for most applications

The E.Z.N.A. Plant DNA DS Kit is designed for the efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen or dried plant tissue samples rich in polysaccharides, polyphenols, or those with a lower DNA content. Up to 50 mg wet tissue (or 15 mg dry tissue) can be processed in less than 1 hour. These systems combine the reversible nucleic acid-binding properties of the matrix with the speed and versatility of spin column technology to eliminate  polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction enzyme digestion and hybridization applications.

These procedures rely on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB-based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB and with binding conditions optimized, DNA is purified using a DNA mini column. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA.

For Research Use Only. Not for use in diagnostic procedures.

Starting AmountUp to 50 mg wet tissue
Starting MaterialFresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content
Elution Volume50-100 μL
TechnologyHiBind® DNA Mini Column
Processing ModeManual
NoteCTAB lysis
HiBind® DNA Mini Columns View Product
Homogenizer Mini Columns View Product
2 mL Collection Tubes View Product
CSPL Buffer View Product
Proteinase K Solution View Product
RBB Buffer View Product
XP2 Binding Buffer View Product
HBC Buffer View Product
DNA Wash Buffer View Product
Elution Buffer View Product
CSPL Buffer View Product
RNase A View Product

DNA Yield Comparison

Figure 1. Comparison of DNA yield from multiple crops. 40-50 mg of respective fresh leaf tissue was extracted in triplicate according to manufacturer’s recommended protocols and eluted in 100 µL. DNA analyzed with fluorescent DNA-based quantification method. Total yield was divided by total tissue amount to show ng of DNA per mg of leaf tissue.

qPCR Comparison

Figure 2. qPCR comparison from corn samples. Real-time PCR with corn-specific primers was performed on triplicates of undiluted, 10-fold and 100-fold dilutions of DNA. DNA was isolated using Omega Bio-tek’s E.Z.N.A.® Plant DNA DS Kit and a comparable column-based kit from Company Q. Omega Bio-tek’s kit not only has significantly higher yields but also less qPCR inhibition when compared to that of Company Q’s.

Yield & Quality

Figure 3. Genomic DNA was purified from either 50 mg potato leaf or 30 mg corn leaf powder with the E.Z.N.A. Plant DNA DS Kit. DNA concentration determined by optical density measurements with NanoDrop® 2000c. Total elution volume was 100 µL.

Size Analysis

Figure 4. Genomic DNA was purified from 50 mg potato leaf with the E.Z.N.A. Plant DNA DS Kit.  5 µL eluate DNA was analyzed on a 1% Agarose gel.

Ct Value Comparison

Figure 5. Genomic DNA was extracted from 50 mg potato leaf and 30 mg corn lead powder using the E.Z.N.A. Plant DNA DS Kit . 2 µL of Eluted DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. C: inhibitor-free control; T: gDNA samples. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors

Product Information

Quick GuideProduct ManualEnglish
Protocol ManualProduct ManualEnglish
E.Z.N.A.® Plant DNA DS KitProduct LiteratureEnglish
Rapid, High Performance and Cost-Effective Plant DNA ExtractionsApplication NoteEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
CSPL BufferGHSEnglishenglishghs
CSPL BufferGHSSpanishspanishghs
CSPL BufferREACHFinnishfinnishreach
CSPL BufferREACHEnglishenglishreach
CSPL BufferREACHDanishdanishreach
CSPL BufferREACHGermangermanreach
CSPL BufferREACHItalianitalianreach
CSPL BufferREACHNorwegiannorwegianreach
CSPL BufferREACHSpanishspanishreach
CSPL BufferREACHSwedishswedishreach
CSPL BufferWHMSEnglishenglishwhms
CSPL BufferREACHFrenchfrenchreach
CSPL BufferWHMSFrenchfrenchwhms
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferWHMSFrenchfrenchwhms
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSFrenchfrenchwhms
Elution BufferWHMSEnglishenglishwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
HBC BufferGHSEnglishenglishghs
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHGermangermanreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHSwedishswedishreach
HBC BufferREACHSpanishspanishreach
HBC BufferWHMSEnglishenglishwhms
HBC BufferWHMSFrenchfrenchwhms
Proteinase K SolutionGHSEnglishenglishghs
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHFinnishfinnishreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHNorwegiannorwegianreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHPortugueseportuguesereach
RBB BufferGHSEnglishenglishghs
RBB BufferGHSSpanishspanishghs
RBB BufferREACHFinnishfinnishreach
RBB BufferREACHDanishdanishreach
RBB BufferREACHEnglishenglishreach
RBB BufferREACHFrenchfrenchreach
RBB BufferREACHGermangermanreach
RBB BufferREACHItalianitalianreach
RBB BufferREACHNorwegiannorwegianreach
RBB BufferREACHSpanishspanishreach
RBB BufferREACHSwedishswedishreach
RBB BufferWHMSEnglishenglishwhms
RBB BufferWHMSFrenchfrenchwhms
XP2 Binding BufferREACHDanishdanishreach
XP2 Binding BufferREACHFrenchfrenchreach
XP2 Binding BufferREACHFinnishfinnishreach
XP2 Binding BufferREACHEnglishenglishreach
XP2 Binding BufferGHSSpanishspanishghs
XP2 Binding BufferGHSEnglishenglishghs
XP2 Binding BufferREACHGermangermanreach
XP2 Binding BufferREACHItalianitalianreach
XP2 Binding BufferREACHNorwegiannorwegianreach
XP2 Binding BufferREACHSpanishspanishreach
XP2 Binding BufferREACHSwedishswedishreach
XP2 Binding BufferWHMSEnglishenglishwhms
XP2 Binding BufferWHMSFrenchfrenchwhms
Elution BufferREACHGreekgreekreach
Proteinase K SolutionREACHGreekgreekreach
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