Using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit

on the Tecan Fluent® Automation Workstation

High-throughput DNA isolation from blood, buccal swabs, saliva & tissue using magnetic beads

  • DNA Extraction from Blood, Saliva, Buffy Coat, Buccal Swabs, Cultured Cells, and Tissues.
  • High Quality DNA is ready for NGS, Microarrays, and qPCR.
  • CE-IVD marked kit available in some markets.

Automated Protocol Specifications

Feature Specification
Sample Amount up to 250 µL Whole Blood; up to 500 µL Saliva
Estimated Offline Processing Time 5 minutes
Online Processing Time 90 minutes Whole Blood; 80 minutes Saliva
Ready to test our chemistry? Request your sample kit today!

Contact us to learn how to get this ready-to-run protocol and script:

M6399 on a Tecan Liquid Handler Information Request
CAPTCHA

Mag-Bind® Blood & Tissue DNA HDQ 96 Kit

High-throughput DNA isolation from blood, buccal swabs, saliva & tissue using magnetic beads

  • DNA Extraction from Blood, Saliva, Buffy Coat, Buccal Swabs, Cultured Cells, and Tissues
  • Automation Friendly Formats allows for up to 384 samples to be processed in 2.5 hours
  • High Quality DNA is ready for NGS, Microarrays, and qPCR
Learn more about the kit:

Mag-Bind® Blood & Tissue DNA HDQ 96 Kit

CE-IVD marked kit is available: Mag-Bind® Blood & Tissue DNA HDQ 96 Kit CE IVD – (omegabiotek.com)

Automated Solution Data

Comparable DNA Yield Regardless of Extraction Method

Figure 1. DNA was extracted from 250 µL of whole blood samples and was eluted in 100 µL volume using the Tecan Fluent 780. The DNA yield was determined using Thermo Scientific’s NanoDrop 2000c and Promega’s QuantiFluor dsDNA system. The average DNA yield from manual and automated extractions were found to be comparable and not significantly different (Tukey’s post-hoc analysis; p>0.05).

Comparable DNA Quality Regardless of Extraction Method

Figure 2. DNA was extracted from 250 µL of whole blood samples and was eluted in 100 µL volume using the Tecan Fluent 780. The purity of DNA isolated using manual and automated protocols was analyzed through spectrophotometry focusing on A260/A280 and A260/A230 ratios.

Automated Extraction of Unfragmented DNA

Figure 3. TapeStation analysis performed on the DNA extracted from 250 μL of blood following protocol automated on Tecan Fluent 780 workstation and protocol performed manually.

Inhibitor-free DNA Extraction

Figure 4. DNA was extracted from 250 µL of whole blood samples and was eluted in 100 µL volume. Average Ct values obtained amplifying the purified DNA following the automated protocol for the Tecan Fluent 780, as well as manual protocols using Omega Bio-tek’s kit.

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

Scripts available for all Mag-Bind® Kits for most open liquid handlers and common magnetic processors including the MagBinder® Fit24. Visit Automated Solutions for more information.

Shopping Cart
Scroll to Top