Mag-Bind® Blood & Tissue DNA HDQ 96 Kit

$0.00$4,900.00

  • DNA Extraction from Blood, Saliva, Buffy Coat, Buccal Swabs, Cultured Cells, and Tissues
  • Automation Friendly Formats allows for up to 384 samples to be processed in 2.5 hours
  • High Quality DNA is ready for NGS, Microarrays, and qPCR

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

The Mag-Bind® Blood & Tissue DNA HDQ 96 Kit offers a versatile solution for the isolation of high-quality DNA from a wide variety of samples, including fresh or frozen cultured cells and tissues, buccal swabs, mouse tail snips, dried blood spots, up to 250 µL whole blood, and up to 500 µL saliva.

Our cutting-edge Mag-Bind® technology reduces overall processing time by providing a quick magnetic response. This Kit combines the reversible nucleic acid-binding properties of Mag-Bind® paramagnetic particles with our proprietary chemistries to provide a rapid and reliable method for DNA isolation from a variety of samples. Purified DNA is high-quality and suitable for use in myriad downstream applications, including PCR, NGS, and enzymatic reactions.

Ready-to-implement protocols are available for the following platforms:

  • Accuris IsoPURE™ 96
  • Hamilton Microlab® STAR
  • Hamilton Microlab® NIMBUS
  • Tecan Fluent®
  • Tecan Freedom EVO®
  • Thermo Fisher Scientific KingFisher® Apex
  • Thermo Fisher Scientific KingFisher® Flex

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting Amount100-250 μL blood samples, saliva, swabs, mouse tails, dried blood spots or 5 x 106 cultured cells.
Elution Volume50-200 µL
TechnologyMagnetic Beads
Processing ModeAutomated, Manual
Throughput8 – 384
NoteDownstream applications: NGS, qPCR, microarray
ITEMAVAILABLE SEPARATELY
Mag-Bind® Particles HDQCall for Pricing
AL BufferView Product
TL BufferView Product
HDQ Binding BufferView Product
VHB BufferView Product
SPM BufferView Product
Proteinase K SolutionView Product
Elution BufferView Product

Yield from Whole Blood

Figure 1.  DNA yield from 200 µL whole blood extracted using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit on the ABI MagMAX 96 instrument. DNA yield was determined by PicoGreen® quantification.

Yield from Buccal Swabs

Figure 2.  Genomic DNA was extracted from buccal swab samples using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit and Company A’s genomic DNA extraction kit. 10% of DNA extracted from each sample was analyzed on a 0.8% agarose gel.

High Quality, Inhibitor-free

Figure 3.  Genomic DNA was extracted from 1,500 µL whole blood using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit. The elution volume was 500 µL. DNA was analyzed on Thermo Scientific’s NanoDrop® 2000c. 50 ng of DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors.

DNA Size

Figure 4.  The sizes of genomic DNA purified using Mag-Bind Blood & Tissue DNA HDQ Kit were determined by electrophoresis.  Sample 1 and 2 were gDNA from human blood.  L is 50 kb genomic ladder for Agilent TapeStation 2200.  The size of gDNA is around 60 kb.

Can the HDQ Binding Buffer and the Mag-Bind Particles HDQ be made into a master mix?

Yes, they can be made into a master mix before starting the experiment.

Do you have a protocol for dried blood spots using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit?

  1. Cut or punch-out the blood spot from the filter paper (up to 200 µL blood can be used per spot). Tear or cut the filter paper into small pieces and place them into a 2 mL nuclease-free microcentrifuge tube.
  2. Add 250 µL PBS.
  3. Incubate at 65 °C for 1 hour. Vortex briefly every 20 minutes.
  4. Add 25 µL Proteinase K Solution. Vortex at maximum speed for 15 seconds.
  5. Incubate at 65 °C for 30 minutes. Vortex briefly several times during incubation.
  6. Centrifuge at ≥13,000 x g for 5 minutes.
  7. Transfer the supernatant to a nuclease-free 2 mL microcentrifuge tube.
  8. Add 290 µL AL Buffer and vortex, or pipet up and down 20 times, to mix. Optional: Add 5 µL RNase A to each sample. Vortex to mix. Let sit at room temperature for 2 minutes.
  9. Continue from step 5 (page 6) of the ‘Blood Protocol’ (250 µL) on the Mag-Bind Blood & Tissue DNA HDQ 96 Kit manual.

Can the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit be used for extraction of blood in PAXgene™ tubes?

Yes. See the protocol modification below. Please be aware we have not validated this protocol.

  1. Pour all the blood from one PAXgene blood DNA tube into a 50 mL centrifuge containing 25 ml NL buffer. Mix by inverting the tube 5 – 10 times.
  2. Centrifuge for 5 minutes at 2500 x g in a swing bucket rotor. Carefully discard the supernatant.
  3. Suspend the pellet in 1 mL of PBS or elution buffer.
  4. Follow the M6399 ‘Blood Protocol’ – either scale up the protocol 4 times or run 4 parallel preps of 250 µL each.

I accidentally left the magnetic beads out when it says to store them in the fridge. Are they ok to use?

The Mag-Bind particles are stable at room temperature for 2 weeks.

Product Information

Document NameLanguagesLink
A Simple, User-Friendly, High Throughput DNA Extraction Workflow Using 2-D Barcoded Buccal Swabs on Hamilton’s Firefly NIMBUS® 96English
Complete High Throughput Pharmacogenomics Workflow: From Sample to ResultsEnglish
Comprehensive, High Throughput Workflow for Automated gDNA Isolation from iSWAB Oral SamplesEnglish
DNA Extraction from Whole Blood on Hamilton’s Microlab® STAR®English
Fully automated DNA extraction solution from Omega Bio-tek using saliva stabilized in Biomatrica’s Salivagard HT DNA collection tubesEnglish
Genomic DNA Normalization Using Omega Bio-tek’s Mag-Bind® EquiPure gDNA Normalization KitEnglish
High Throughput Workflows for DNA Extractions from Oral SamplesEnglish
High Throughput, Automated DNA Extraction Solution from Whole Blood Samples Using Omega Bio-tek’s Reagents on Tecan Fluent 780 WorkstationEnglish
M6399 Mag-Bind Blood & Tissue DNA HDQ 96 Kit SL-0014English
Mag-Bind® Blood & Tissue DNA HDQ 96 KitEnglish
Protocol ManualEnglish
Quick GuideEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
AL BufferGHSEnglishenglishghs
AL BufferGHSSpanishspanishghs
AL BufferREACHEnglishenglishreach
AL BufferREACHDanishdanishreach
AL BufferREACHFinnishfinnishreach
AL BufferREACHFrenchfrenchreach
AL BufferREACHGermangermanreach
AL BufferREACHItalianitalianreach
AL BufferREACHNorwegiannorwegianreach
AL BufferREACHSpanishspanishreach
AL BufferREACHSwedishswedishreach
AL BufferWHMSEnglishenglishwhms
AL BufferWHMSFrenchfrenchwhms
AL BufferREACHDutchdutchreach
AL BufferREACHHungarianhungarianreach
AL BufferREACHPortugueseportuguesereach
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
HDQ Binding BufferGHSEnglishenglishghs
HDQ Binding BufferGHSSpanishspanishghs
HDQ Binding BufferREACHEnglishenglishreach
HDQ Binding BufferREACHDanishdanishreach
HDQ Binding BufferREACHFinnishfinnishreach
HDQ Binding BufferREACHFrenchfrenchreach
HDQ Binding BufferREACHGermangermanreach
HDQ Binding BufferREACHItalianitalianreach
HDQ Binding BufferREACHNorwegiannorwegianreach
HDQ Binding BufferREACHSpanishspanishreach
HDQ Binding BufferREACHSwedishswedishreach
HDQ Binding BufferWHMSEnglishenglishwhms
HDQ Binding BufferWHMSFrenchfrenchwhms
HDQ Binding BufferREACHDutchdutchreach
HDQ Binding BufferREACHHungarianhungarianreach
HDQ Binding BufferREACHPortugueseportuguesereach
Mag-Bind Particles HDQGHSEnglishenglishghs
Mag-Bind Particles HDQREACHEnglishenglishreach
Mag-Bind Particles HDQREACHDanishdanishreach
Mag-Bind Particles HDQREACHFinnishfinnishreach
Mag-Bind Particles HDQREACHFrenchfrenchreach
Mag-Bind Particles HDQREACHGermangermanreach
Mag-Bind Particles HDQREACHItalianitalianreach
Mag-Bind Particles HDQREACHNorwegiannorwegianreach
Mag-Bind Particles HDQREACHSpanishspanishreach
Mag-Bind Particles HDQREACHSwedishswedishreach
Mag-Bind Particles HDQWHMSEnglishenglishwhms
Mag-Bind Particles HDQWHMSFrenchfrenchwhms
Mag-Bind Particles HDQREACHDutchdutchreach
Mag-Bind Particles HDQREACHHungarianhungarianreach
Mag-Bind Particles HDQREACHPortugueseportuguesereach
Proteinase K SolutionGHSEnglishenglishghs
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHFinnishfinnishreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionREACHNorwegiannorwegianreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHPortugueseportuguesereach
SPM BufferGHSEnglishenglishghs
SPM BufferGHSSpanishspanishghs
SPM BufferREACHEnglishenglishreach
SPM BufferREACHDanishdanishreach
SPM BufferREACHFinnishfinnishreach
SPM BufferREACHFrenchfrenchreach
SPM BufferREACHGermangermanreach
SPM BufferREACHItalianitalianreach
SPM BufferREACHNorwegiannorwegianreach
SPM BufferREACHSpanishspanishreach
SPM BufferREACHSwedishswedishreach
SPM BufferWHMSEnglishenglishwhms
SPM BufferWHMSFrenchfrenchwhms
SPM BufferREACHDutchdutchreach
SPM BufferREACHHungarianhungarianreach
SPM BufferREACHPortugueseportuguesereach
TL BufferGHSEnglishenglishghs
TL BufferGHSSpanishspanishghs
TL BufferREACHEnglishenglishreach
TL BufferREACHDanishdanishreach
TL BufferREACHFinnishfinnishreach
TL BufferREACHFrenchfrenchreach
TL BufferREACHGermangermanreach
TL BufferREACHItalianitalianreach
TL BufferREACHNorwegiannorwegianreach
TL BufferREACHSpanishspanishreach
TL BufferREACHSwedishswedishreach
TL BufferWHMSEnglishenglishwhms
TL BufferWHMSFrenchfrenchwhms
TL BufferREACHDutchdutchreach
TL BufferREACHHungarianhungarianreach
TL BufferREACHPortugueseportuguesereach
VHB BufferGHSEnglishenglishghs
VHB BufferGHSSpanishspanishghs
VHB BufferREACHEnglishenglishreach
VHB BufferREACHDanishdanishreach
VHB BufferREACHFinnishfinnishreach
VHB BufferREACHFrenchfrenchreach
VHB BufferREACHGermangermanreach
VHB BufferREACHItalianitalianreach
VHB BufferREACHNorwegiannorwegianreach
VHB BufferREACHSpanishspanishreach
VHB BufferREACHSwedishswedishreach
VHB BufferWHMSEnglishenglishwhms
VHB BufferWHMSFrenchfrenchwhms
VHB BufferREACHDutchdutchreach
VHB BufferREACHHungarianhungarianreach
VHB BufferREACHPortugueseportuguesereach
Shopping Cart
Scroll to Top