Automated gDNA Isolation from Swabs, Tissues, Blood, Saliva, and Cells
on the MagBinder® Fit24
High-throughput DNA isolation from blood, buccal swabs, saliva & tissue using magnetic beads
- DNA Extraction from Blood, Saliva, Buffy Coat, Buccal Swabs, Cultured Cells, and Tissues
- High Quality DNA is ready for NGS, Microarrays, and qPCR
Automated Protocol Specifications
| Feature | Specification |
|---|---|
| Input Amount | 250 μL Blood, 500 μL Saliva, 10 mg Tissue, 1 x 106 Cells, or Buccal Swab |
| Throughput | 1-24 Samples |
| Estimated Offline Processing Time | Blood, Saliva, Cells, Swab: 30 minutes, Tissue: 1 to 3 hours |
| Online Processing Time | 43 minutes |
Contact us to learn how to get this ready-to-run protocol and script:
Mag-Bind® Blood & Tissue DNA HDQ 96 Kit
High-throughput DNA isolation from blood, buccal swabs, saliva & tissue using magnetic beads
- DNA Extraction from Blood, Saliva, Buffy Coat, Buccal Swabs, Cultured Cells, and Tissues
- Automation Friendly Formats allows for up to 384 samples to be processed in 2.5 hours
- High Quality DNA is ready for NGS, Microarrays, and qPCR
CE-IVD marked kit is available!
Learn more about the kit:
Automated Solution Data
High-Yielding DNA with Excellent Purity Ratios from Buffy Coat
Figure 1. Genomic DNA was extracted from 100 µL buffy coat using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit automated on the MagBinder® Fit24. Yield and quality were analyzed via Thermo Scientific’s NanoDrop® 2000c. The results indicate consistent high-yielding DNA with excellent purity ratios.
Unfragmented, High Integrity DNA from Buffy Coat
Figure 2. TapeStation® analysis of purified DNA from 100 µL buffy coat showing high molecular weight DNA >60 kb and high DIN scores (>9.2) indicating high integrity intact DNA extracted using Mag-Bind® Blood & Tissue DNA HDQ 96 Kit automated on the MagBinder® Fit24.
Inhibition-free and Efficient Downstream qPCR from Buffy Coat
Figure 3. Average Ct values obtained on 10X and 100X dilutions of purified DNA extracted using Mag-Bind® Blood & Tissue DNA HDQ 96 Kit automated on the MagBinder® Fit24. The Ct values differed by ~ 3.3 cycles per 10-fold dilution indicating inhibition-free and efficient downstream qPCR analysis.