Using the Mag-Bind® Endo-free Plasmid Midi Kit

on the MagBinder® Fit24

The Mag-Bind® Endo-free Plasmid Midi Kit (M1272) extracts high-yielding, high-quality plasmid DNA from a wide range of plasmid types and culture media:

  • High-yielding – Plasmid DNA yield up to 200 μg
  • Versatile – Process up to 50 mL bacterial culture with different plasmid types and culture medias
  • Transfection-quality – Endotoxin free plasmid DNA with endotoxins levels < 0.1 EU/µg
  • Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8

Automated Protocol Specifications

Feature Specification
Input Amount up to 50 mL LB or 10 TB Culture
Throughput 1-24 Samples
Estimated Offline Processing Time 40 minutes
Online Processing Time 64 minutes
Ready to test our chemistry? Request your sample kit today!

Contact us to learn how to get this ready-to-run protocol and script:

M1272 on a MagBinder Information Request
CAPTCHA

Mag-Bind® Endo-free Plasmid Midi Kit

Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 50 mL culture volume.

  • Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
  • High-yielding – Plasmid DNA yield up to 200 μg
  • Rapid – 24 samples can be processed in ~ 70 minutes starting from a bacterial pellet without manual intervention
  • Versatile – Process up to 50 mL bacterial culture with different plasmid types and culture medias
  • Transfection-quality – Endotoxin free plasmid DNA with endotoxins levels < 0.1 EU/µg
  • Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8
Learn more about the kit:

Mag-Bind® Endo-free Plasmid Midi Kit

 

Automated Solution Data

Significantly higher Plasmid yields compared to Company T

Figure 1. pGEM and pGL4.51 plasmids were purified from 5 mL DH5α cultures (n=3) using the Mag-Bind® Endo-free Plasmid Midi Kit on the MagBinder® Fit24 Nucleic Acid Purification System, as well as Company T’s Kit following manufacturers’ recommended centrigation protocols. Purified Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system. Plasmid purification was not only endotoxin-free (<0.1 EU/μg) for Omega Bio-tek’s Kit, but the yield was also significantly higher compared to Company T’s Kit.

Higher transfection efficiency vs. Company T

Figure 2. Plasmid pGL4.51 was prepared from 5 mL DH5α cultures (n=3) and purified using the Mag-Bind® Endo-free Plasmid Midi Kit on the MagBinder® Fit24 Nucleic Acid Purification System, as well as Company T’s equivalent Kit. Purified Plasmids were transfected into 10,000 cells with 200 ng of purified plasmid using 2 μL Lipofectamine Transfection Reagent. Plasmids purified using Omega Bio-tek’s Kit resulted in higher bioluminscent signal, indicating higher transfection efficiency compared to plasmids purified using Company T’s Kit.

Excellent Plasmid Yields and Purity Extracted on MagBinder® Fit24 Platform

Figure 3. pGEM plasmid was purified from 25 mL and 50 mL LB as well as 10 mL TB media (n=3) using Mag-Bind® Endo-free Plasmid Midi Kit automated on the MagBinder® Fit24 Platform following three different lysate clearance methodologies. Yields and quality were comparable, irrespective of the lysate clearance methodology used.

From Automated Plasmid Purification to In Vitro Transcription and mRNA Purification: Use Case for Early Discovery of mRNA-based Therapeutics

Kiranmai Durvasula¹, Carisa Townsend¹, Julie Baggs¹, and Travis Butts¹ ¹Omega Bio-tek, Inc, Norcross, GA 30071 Introduction Plasmids play a crucial role in the manufacturing and development of mRNA-based vaccines and therapeutics. As essential tools in molecular biology, plasmids serve as templates for in vitro transcription (IVT) processes, enabling the production of high-quality mRNA molecules for

Read More »
Shopping Cart
Scroll to Top