$0.00 – $385.30
Purify endotoxin-free plasmid DNA (<0.1 EU/µg) using magnetic beads
The Mag-Bind® Endo-free Plasmid Mini Kit and the Mag-Bind® Endo-free Plasmid Midi Kit are recommended for new projects. Sample kits are available!
All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.
Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.
Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.
The Mag-Bind® family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. Key to the system is Omega Bio-tek’s proprietary Mag-Bind® Particle that avidly, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer.
The Mag-Bind® Ultra-Pure Plasmid DNA 96 Kit combines the power of Mag-Bind® technology with the innovative ETR technology (Endo-toxin removal) to deliver high-quality endotoxin-free plasmid DNA in high throughput format. Yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 1 mL of overnight culture in LB medium typically produces 10-15 μg for high-copy plasmid. The purified plasmid can be used directly for automated fluorescent DNA sequencing, such as with BigDye® sequencing chemistry, transfection as well as for other standard molecular biology techniques including restriction enzyme digestion.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | Sensitive cell line transfection; cloning; sanger sequencing etc. |
| Starting material | Per well: 1.0-1.5 mL LB culture with OD600 between 2 and 3; or equivalent |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing mode | Automated, Magnetic processing |
| Throughput | 96 samples per run |
| DNA binding technology | Magnetic beads |
| Lysate clearance method | Centrifugation, Magnetic Beads or 96-well Filter Plate |
| Yield | 10-15 μg for high copy-number plasmid |
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| Mag-Bind® Particles RQ | Call for Pricing |
| Solution I | View Product |
| Solution II | View Product |
| N3 Buffer | — |
| ETR Binding Buffer | View Product |
| ETR Wash Buffer | |
| VHB Buffer | View Product |
| SPM Wash Buffer | View Product |
| Elution Buffer | View Product |
| RNase A | View Product |
Figure 1. 9 samples of 1 mL E. Coli culture transformed with pGEM vector were purified using the Mag-Bind® Ultra-Pure Plasmid Kit. The elution volume was 100 µL. The DNA concentration was measured with Nanodrop 2000c.
Figure 2. 1 mL DH5α cultures transformed with pGEM vector were isolated according to the standard protocol. 5 µL eluate plasmid DNA was analyzed on a 1% Agarose gel.
Figure 3. Plasmid DNA purified with Mag-Bind Ultra-Pure Plasmid DNA Kit was used in a 5 µL Sanger sequencing reaction. DNA was analyzed on an Applied Biosystems 3730XL.
Figure 4. Endotoxins in plasmid DNA preps. Plasmid DNA was isolated from 0.8 mL LB cultures following each manufacturer’s recommended protocols. Endotoxin levels were determined with Thermo Scientific’s Pierce LAL Chromogenic Endotoxin Quantitation Kit.
