Mag-Bind® Universal Pathogen 96 Kit

on the Hamilton Microlab® STAR™

High-throughput DNA & viral RNA isolation from a variety of sample sources

  • Ceramic Beads Pre-Aliquoted to 96-well Plates
  • Isolates Yeast, Fungal, Bacterial, and Viral DNA
  • Isolate Viral RNA

Automated Protocol Specifications

Feature Specification
Input Amount up to 250 µL
Estimated Offline Processing Time 30 minutes
Online Processing Time 60 minutes

Contact us to learn how to get this ready-to-run protocol and script:

M4029 on a Hamilton Liquid Handler Information Request
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Mag-Bind® Universal Pathogen 96 Kit

High-throughput DNA & viral RNA isolation from a variety of sample sources

  • Ceramic Beads Pre-Aliquoted to 96-well Plates
  • Isolates Yeast, Fungal, Bacterial, and Viral DNA
  • Isolate Viral RNA

Available with or without disrupter plates!

Learn more about the kit:

Mag-Bind® Universal Pathogen 96 Kit

 

Automated Solution Data

Inhibitor-free Gram-Positive Bacterial DNA Isolation from Stool Samples

Figure 1. Gram Positive Bacteria Bacillus subtilis was spiked into 250 µL stool samples and isolated using the Mag-Bind® Universal Pathogen 96 Kit. Automated isolation was performed using the Hamilton Firefly Nimbus® 96 and compared to manual isolation methods. qPCR was performed on undiluted, 10-fold, and 100-fold dilutions. The ΔCt across dilutions was ~3.3 for both manual and automated methods, indicating minimal inhibition.

Comparable Yields Between Manual and Automated Extraction Methods for Stool Samples

Figure 2. Yeast Saccharomyces cerevisiae was spiked into 250 µL stool samples and isolated using the Mag-Bind® Universal Pathogen 96 Kit. Automated isolation was performed using the Hamilton FireFly Nimbus 96 and compared to manual isolation methods. Quantification showed comparable yields of yeast DNA between manual and automated isolation methods.

Inhibitor-free Yeast DNA Isolation from Stool Samples

Figure 3. Yeast Saccharomyces cerevisiae was spiked into 250 µL stool samples and isolated using the Mag-Bind® Universal Pathogen 96 Kit. Automated isolation was performed using the Hamilton FireFly Nimbus 96 and compared to manual isolation methods. qPCR was performed on undiluted, 10-fold, and 100-fold dilutions. The ΔCt across dilutions was ~3.3 for both manual and automated methods, indicating minimal inhibition.

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

Scripts available for all Mag-Bind® Kits for most open liquid handlers and common magnetic processors including the MagBinder® Fit24. Visit Automated Solutions for more information.

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