E.Z.N.A.® Plasmid DNA Midi Kit
$0.00 – $682.10
- Speed – Purification of plasmid DNA in < 60 minutes
- Safety – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High quality – DNA is suitable for a variety of downstream applications
The E.Z.N.A. Plasmid Midi and Maxi Kits can isolate up to 250 μg and 1.2 mg of plasmid DNA from 50 mL and 200 mL bacterial cultures, respectively. These kits use a modified alkaline lysis method to lyse the cells and separate genomic DNA from plasmid DNA. Cellular debris are removed by centrifugation, and the protocol follows a simple bind, wash, and elute procedure to deliver high-quality
plasmid DNA. The system not only eliminates the time-consuming isopropanol precipitation step required by gravity flow columns but also the need for expensive accessories. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR, in-vitro transcription, transformation, and other applications.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc. |
| Starting material | 15-50 mL LB culture; Optimal Cell Mass (OD600 x mL culture) is 80-100. |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing mode | Manual (centrifugation or vacuum) |
| Throughput | 1 – 24 |
| DNA binding technology | Silica Midi Spin Column |
| Lysate clearance method | Centrifugation |
| Processing time | <60 minutes |
| Yield | 100-250 µg for high copy-number; 10-50 µg for low copy-number |
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| HiBind® DNA Mini Columns | View Product |
| 15 mL Collection Tubes | — |
| Solution I | View Product |
| Solution II | View Product |
| Solution III | View Product |
| HBC Buffer | View Product |
| DNA Wash Buffer | View Product |
| RNase A | View Product |
| Elution Buffer | View Product |
Table 1. pGEM plasmid was purified from 50 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Midi Kit following manufacturer’s recommended protocol. Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system.