E.Z.N.A.® Plasmid DNA Maxi Kit


  • Speed – Purification of plasmid DNA in < 60 minutes
  • Safety – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High quality – DNA is suitable for a variety of downstream applications

The E.Z.N.A.® Plasmid DNA Maxi Kit combines the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiBind® DNA columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be processed simultaneously. Up to 600-1200 µg high copy number plasmid DNA or 50-300 µg low copy number plasmid DNA can be purified from 50-200 mL overnight culture using E.Z.N.A.® Plasmid DNA Maxi Kit. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationCloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting materialOptimal volume depends on culture density and plasmid copy number. The optimal cell mass (OD600 x mL culture) is 80-100.
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeManual (centrifugation or vacuum)
Throughput1 – 24
DNA binding technologySilica Maxi Spin Column
Lysate clearance methodCentrifugation
Processing time<60 minutes
Yield600-1200 µg for high copy-number; 50-300 µg for low copy-number
HiBind® DNA Maxi Columns View Product
50 mL Collection Tubes
Solution I View Product
Solution II View Product
Solution III View Product
HBC Buffer View Product
DNA Wash Buffer View Product
RNase A View Product
Elution Buffer View Product

Figure 1.  pGEM plasmid was purified from 250 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Maxi Kit following manufacturer’s recommended protocols. Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system.

Product Information

Document NameLanguagesLink
E.Z.N.A.® Plasmid DNA Midi & Maxi KitsEnglish
Protocol ManualEnglish
Quick GuideEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHDutchdutchreach
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHSwedishswedishreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHGermangermanreach
Elution BufferREACHFrenchfrenchreach
Elution BufferGHSEnglishenglishghs
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHEnglishenglishreach
Elution BufferGHSSpanishspanishghs
Elution BufferREACHItalianitalianreach
HBC BufferREACHGermangermanreach
HBC BufferWHMSFrenchfrenchwhms
HBC BufferWHMSEnglishenglishwhms
HBC BufferREACHSwedishswedishreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHEnglishenglishreach
HBC BufferGHSSpanishspanishghs
HBC BufferGHSEnglishenglishghs
RNase AWHMSFrenchfrenchwhms
RNase AWHMSEnglishenglishwhms
RNase AREACHSwedishswedishreach
RNase AREACHSpanishspanishreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHFrenchfrenchreach
RNase AREACHItalianitalianreach
RNase AREACHFinnishfinnishreach
RNase AREACHDanishdanishreach
RNase AREACHEnglishenglishreach
RNase AGHSSpanishspanishghs
RNase AGHSEnglishenglishghs
RNase AREACHGermangermanreach
Solution IREACHItalianitalianreach
Solution IWHMSFrenchfrenchwhms
Solution IWHMSEnglishenglishwhms
Solution IREACHSwedishswedishreach
Solution IREACHSpanishspanishreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHGermangermanreach
Solution IREACHFrenchfrenchreach
Solution IREACHFinnishfinnishreach
Solution IREACHDanishdanishreach
Solution IREACHEnglishenglishreach
Solution IGHSSpanishspanishghs
Solution IGHSEnglishenglishghs
Solution IIREACHDanishdanishreach
Solution IIWHMSFrenchfrenchwhms
Solution IIWHMSEnglishenglishwhms
Solution IIREACHSwedishswedishreach
Solution IIREACHSpanishspanishreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHItalianitalianreach
Solution IIREACHFrenchfrenchreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHEnglishenglishreach
Solution IIGHSSpanishspanishghs
Solution IIGHSEnglishenglishghs
Solution IIREACHGermangermanreach
Solution IIIWHMSFrenchfrenchwhms
Solution IIIWHMSEnglishenglishwhms
Solution IIIREACHSwedishswedishreach
Solution IIIREACHSpanishspanishreach
Solution IIIREACHNorwegiannorwegianreach
Solution IIIREACHItalianitalianreach
Solution IIIREACHGermangermanreach
Solution IIIREACHFrenchfrenchreach
Solution IIIREACHFinnishfinnishreach
Solution IIIREACHDanishdanishreach
Solution IIIREACHEnglishenglishreach
Solution IIIGHSSpanishspanishghs
Solution IIIGHSEnglishenglishghs
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