E.Z.N.A.® Plasmid DNA Maxi Kit


  • Speed – Purification of plasmid DNA in < 60 minutes
  • Safety – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High quality – DNA is suitable for a variety of downstream applications

The E.Z.N.A.® Plasmid DNA Maxi Kit combines the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiBind® DNA columns facilitate the binding, washing, and elution steps, thus enabling multiple samples to be processed simultaneously. Up to 600-1200 µg high copy number plasmid DNA or 50-300 µg low copy number plasmid DNA can be purified from 50-200 mL overnight culture using E.Z.N.A.® Plasmid DNA Maxi Kit. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations.

The E.Z.N.A. Plasmid Maxi Kit can isolate up to 250 μg and 1.2 mg of plasmid DNA from 50 mL and 200 mL bacterial cultures, respectively. The kit uses a modified alkaline lysis method to lyse the cells and separate genomic DNA from plasmid DNA. Cellular debris are removed by centrifugation, and the protocol follows a simple bind, wash, and elute procedure to deliver high-quality plasmid DNA. The system not only eliminates the time-consuming isopropanol precipitation step required by gravity flow columns but also the need for expensive accessories.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationCloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting materialOptimal volume depends on culture density and plasmid copy number. The optimal cell mass (OD600 x mL culture) is 80-100.
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeManual (centrifugation or vacuum)
Throughput1 – 24
DNA binding technologySilica Maxi Spin Column
Lysate clearance methodCentrifugation
Processing time<60 minutes
Yield600-1200 µg for high copy-number; 50-300 µg for low copy-number
HiBind® DNA Maxi Columns View Product
50 mL Collection Tubes
Solution I View Product
Solution II View Product
Solution III View Product
HBC Buffer View Product
DNA Wash Buffer View Product
RNase A View Product
Elution Buffer View Product

Figure 1.  pGEM plasmid was purified from 250 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Maxi Kit following manufacturer’s recommended protocols. Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system.

Product Information

Document NameLanguagesLink
Quick GuideEnglish
Protocol ManualEnglish
E.Z.N.A.® Plasmid DNA Midi & Maxi KitsEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferWHMSFrenchfrenchwhms
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
HBC BufferGHSEnglishenglishghs
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHGermangermanreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHSwedishswedishreach
HBC BufferWHMSEnglishenglishwhms
HBC BufferWHMSFrenchfrenchwhms
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHFrenchfrenchreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
Solution IREACHFinnishfinnishreach
Solution IREACHDanishdanishreach
Solution IREACHEnglishenglishreach
Solution IGHSSpanishspanishghs
Solution IGHSEnglishenglishghs
Solution IREACHFrenchfrenchreach
Solution IREACHGermangermanreach
Solution IREACHItalianitalianreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHSpanishspanishreach
Solution IREACHSwedishswedishreach
Solution IWHMSEnglishenglishwhms
Solution IWHMSFrenchfrenchwhms
Solution IIGHSEnglishenglishghs
Solution IIGHSSpanishspanishghs
Solution IIREACHEnglishenglishreach
Solution IIREACHItalianitalianreach
Solution IIREACHGermangermanreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHDanishdanishreach
Solution IIREACHFrenchfrenchreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSpanishspanishreach
Solution IIREACHSwedishswedishreach
Solution IIWHMSEnglishenglishwhms
Solution IIWHMSFrenchfrenchwhms
Solution IIIGHSEnglishenglishghs
Solution IIIREACHDanishdanishreach
Solution IIIREACHGermangermanreach
Solution IIIREACHFrenchfrenchreach
Solution IIIREACHFinnishfinnishreach
Solution IIIREACHEnglishenglishreach
Solution IIIGHSSpanishspanishghs
Solution IIIREACHItalianitalianreach
Solution IIIREACHNorwegiannorwegianreach
Solution IIIREACHSpanishspanishreach
Solution IIIREACHSwedishswedishreach
Solution IIIWHMSEnglishenglishwhms
Solution IIIWHMSFrenchfrenchwhms
Elution BufferREACHGreekgreekreach
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