Downstream Applications

Automated DNA Purification Solution from 2 mL Whole Blood Using Omega Bio-tek’s Reagents on the MagBinder® Fit24 Platform

Leave a Comment / Application Notes, Biobanking, Downstream Applications / By

Carisa Townsend1, Kiranmai Durvasula1, and Travis Butts1 Omega Bio-tek, Inc, Norcross, GA 30071 Introduction With advances in genomics research, personalized medicine, and sequencing-based technologies, there is a necessity for purification of high-quality genomic DNA from large volumes of blood. The rapidly growing landscape of biorepositories that store large amounts of DNA from an enormous number […]

Automated DNA Purification Solution from 2 mL Whole Blood Using Omega Bio-tek’s Reagents on the MagBinder® Fit24 Platform Read More »

RNA Depletion in Plasmid DNA Extractions with Mag-Bind® TotalPure NGS

Clean Up, Downstream Applications, Product Application / By

Introduction Traditionally, plasmid DNA purification hinges on alkaline lysis. In this method, bacteria, typically E. coli, containing the targeted plasmid DNA are harvested from overnight culture and resuspended in solution. A highly alkaline solution containing a detergent lyses the bacteria cells and denatures both the plasmid and genomic DNA. The addition of a highly acidic

RNA Depletion in Plasmid DNA Extractions with Mag-Bind® TotalPure NGS Read More »

DNA from Saliva Performs Well Downstream, Despite Poor A260/A230 Ratios

Downstream Applications, Product Application / By

The non-invasive nature of sample collection makes saliva an appealing sample type for patients and researchers alike. However, one hindrance to using saliva for genomic analysis is the appearance of low purity in eluates when quantified using absorbance ratios. Here, we discuss the nature of absorbance ratios and contextualize their meaning when analyzing extractions from

DNA from Saliva Performs Well Downstream, Despite Poor A260/A230 Ratios Read More »

Shopping Cart
Scroll to Top