Author name: Claire McClain

RNA Depletion in Plasmid DNA Extractions with Mag-Bind® TotalPure NGS

Introduction Traditionally, plasmid DNA purification hinges on alkaline lysis. In this method, bacteria, typically E. coli, containing the targeted plasmid DNA are harvested from overnight culture and resuspended in solution. A highly alkaline solution containing a detergent lyses the bacteria cells and denatures both the plasmid and genomic DNA. The addition of a highly acidic […]

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DNA from Saliva Performs Well Downstream, Despite Poor A260/A230 Ratios

The non-invasive nature of sample collection makes saliva an appealing sample type for patients and researchers alike. However, one hindrance to using saliva for genomic analysis is the appearance of low purity in eluates when quantified using absorbance ratios. Here, we discuss the nature of absorbance ratios and contextualize their meaning when analyzing extractions from

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A Guide to Magnetic Bead-Based Nucleic Acid Extraction

Among the myriad techniques for nucleic acid extraction, magnetic beads stand out as an efficient and reliable tool to implement when automating extraction workflows. Magnetic bead-based extraction relies on the use of paramagnetic particles with coatings capable of specifically binding to nucleic acids. These beads only display magnetic properties when an external magnetic field is

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The Challenges of cfDNA Purification

Cell-free DNA (cfDNA) has emerged as a revolutionary tool in the realm of molecular diagnostics, offering a non-invasive window into the genetic information of various bodily tissues. As discussed in a previous post, the development of cfDNA technology is a large step forward in detecting and profiling circulating biomarkers to derive clinically relevant information. However,

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DNA Sequencing Could Help Restore Genomic Diversity to at-risk Populations of Pumas in California

Introduction The consequences of the expansion of human development have been especially felt in California where human activity has eradicated several apex predators, like the California Grizzly and gray wolf. Unlike these predators, the puma, also called the mountain lion or cougar, has continued to populate the state, though their population is threatened by the continued

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What is PCR?

Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify and replicate specific segments of DNA. Over time, PCR technology has evolved, giving rise to various methodologies, each tailored to specific needs. In this blog post, we will explore the different types of PCR and their applications.  Standard PCR Conventional PCR enables the

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What is NGS?

Next-Generation Sequencing (NGS) is a cutting-edge technology used to sequence nucleotides in a DNA or RNA molecule. Unlike Sanger Sequencing, which can sequence only one DNA fragment at a time, NGS allows researchers to sequence multiple DNA fragments in parallel in a single run. This high-throughput approach revolutionizes our ability to decode complex genomes efficiently

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MagXtract® 3200 Application Showcase: Automated extraction of bacterial DNA from stool samples using Omega Bio-Tek’s Mag-Bind® Universal Pathogen 96 Kit

Victor Chen1, Kiranmai Durvasula2 1. Chroma ATE, Inc, Guishan Dist., Taoyuan City, Taiwan 333001 2. Omega Bio-tek, Inc, Norcross GA 30071 Introduction Stool sample analysis offers a simple, non-invasive methodology for detection of multiple disease-causing microbes, biomarker detection of cancers of the digestive tract to analysis of gut microbiome and their impact on physiological health

MagXtract® 3200 Application Showcase: Automated extraction of bacterial DNA from stool samples using Omega Bio-Tek’s Mag-Bind® Universal Pathogen 96 Kit Read More »

High-Throughput, Fully Automated Cell-Free DNA Extraction from up to 10 mL Serum Samples

Authors: Chris Lee1, Jonatan Lysen1, Jeff Roeder1, Julie Baggs1, Kiranmai Durvasula1, Travis Butts1 Omega Bio-tek, Norcorss, GA 30071 Overview Purpose Development of a fully automated solution to extract cfDNA from up to 10 mL samples in 2.75 hours when integrated on Hamilton’s Microlab® STAR™ platform. Methods cfDNA extracted using automated protocol with Mag-Bind® cfDNA Kit

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