RNA Depletion in Plasmid DNA Extractions with Mag-Bind® TotalPure NGS
Introduction Traditionally, plasmid DNA purification hinges on alkaline lysis. In this method, bacteria, typically E. coli, containing the targeted plasmid DNA are harvested from overnight culture and resuspended in solution. A highly alkaline solution containing a detergent lyses the bacteria cells and denatures both the plasmid and genomic DNA. The addition of a highly acidic […]
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