The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is Omega Bio-tek’s HiBind® matrix that specifically, but reversibly, binds DNA or RNA under optimal conditions while allowing proteins and other contaminants to be removed. The nucleic acids bound to the HiBind® matrix are easily eluted with deionized water or low salt buffer. DNA and RNA are suitable for many downstream applications.
Omega Bio-tek’s E.Z.N.A.® M13 DNA Kits are designed to purify up to 10 µg of single stranded DNA from up to 3 mL of phage supernatant. Yields of single stranded DNA obtained using E.Z.N.A.® M13 DNA Kits are around 3-10 µg and reproducible when the isolations are performed from the same culture.
For Research Use Only. Not for use in diagnostic procedures.
FEATURES | SPECIFICATIONS |
---|---|
Downstream Application | Single-stranded M13 DNA |
Starting material | 3 mL phage supernatant |
Plasmid type | Single-stranded M13 DNA |
Processing mode | Manual, centrifugation |
Throughput | 1 – 24 |
DNA binding technology | Silica Mini Spin Column |
Lysate clearance method | Not needed |
Yield | 3-10 µg single-stranded DNA |
Figure 1. DNA purified using the E.Z.N.A.® M13 DNA Kit. DNA was isolated from 4 1 mL M13 supernatant cultures grown 6 hours in E. coli culture using the E.Z.N.A.® M13 DNA Kit and eluted in 50 µL TE buffer. M13 ssDNA (5% of total purified DNA) was analyzed on a 1.0% agarose gel. Lanes 1-4: M13 ssDNA, M: 1 kb DNA formaldehyde agarose gel..
Product Information
Document Name | Languages | Link |
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Protocol Manual | English |
Safety Data Sheets
Components | Hazard Standards | Languages | Link | hf:tax:dlp_document_language | hf:tax:dlp_document_hazard-standard |
---|---|---|---|---|---|
Elution Buffer | GHS | Spanish | spanish | ghs | |
Elution Buffer | REACH | Greek | greek | reach | |
Elution Buffer | GHS | English | english | ghs | |
Elution Buffer | REACH | Portuguese | portuguese | reach | |
Elution Buffer | REACH | Hungarian | hungarian | reach | |
Elution Buffer | REACH | Dutch | dutch | reach | |
Elution Buffer | WHMS | French | french | whms | |
Elution Buffer | WHMS | English | english | whms | |
Elution Buffer | REACH | Swedish | swedish | reach | |
Elution Buffer | REACH | Spanish | spanish | reach | |
Elution Buffer | REACH | Norwegian | norwegian | reach | |
Elution Buffer | REACH | Italian | italian | reach | |
Elution Buffer | REACH | German | german | reach | |
Elution Buffer | REACH | French | french | reach | |
Elution Buffer | REACH | Finnish | finnish | reach | |
Elution Buffer | REACH | Danish | danish | reach | |
Elution Buffer | REACH | English | english | reach | |
MPG Buffer | REACH | English | english | reach | |
MPG Buffer | WHMS | French | french | whms | |
MPG Buffer | WHMS | English | english | whms | |
MPG Buffer | REACH | Swedish | swedish | reach | |
MPG Buffer | REACH | Spanish | spanish | reach | |
MPG Buffer | REACH | Norwegian | norwegian | reach | |
MPG Buffer | REACH | Italian | italian | reach | |
MPG Buffer | REACH | German | german | reach | |
MPG Buffer | REACH | French | french | reach | |
MPG Buffer | REACH | Finnish | finnish | reach | |
MPG Buffer | REACH | Danish | danish | reach | |
MPG Buffer | GHS | Spanish | spanish | ghs | |
MPG Buffer | GHS | English | english | ghs | |
MPX Buffer | GHS | Spanish | spanish | ghs | |
MPX Buffer | WHMS | French | french | whms | |
MPX Buffer | WHMS | English | english | whms | |
MPX Buffer | REACH | Swedish | swedish | reach | |
MPX Buffer | REACH | Spanish | spanish | reach | |
MPX Buffer | REACH | Norwegian | norwegian | reach | |
MPX Buffer | REACH | German | german | reach | |
MPX Buffer | REACH | French | french | reach | |
MPX Buffer | REACH | Finnish | finnish | reach | |
MPX Buffer | REACH | Danish | danish | reach | |
MPX Buffer | REACH | English | english | reach | |
MPX Buffer | REACH | Italian | italian | reach | |
MPX Buffer | GHS | English | english | ghs | |
SPW Buffer | REACH | Finnish | finnish | reach | |
SPW Buffer | REACH | Greek | greek | reach | |
SPW Buffer | WHMS | French | french | whms | |
SPW Buffer | WHMS | English | english | whms | |
SPW Buffer | GHS | Spanish | spanish | ghs | |
SPW Buffer | GHS | English | english | ghs | |
SPW Buffer | REACH | Swedish | swedish | reach | |
SPW Buffer | REACH | Spanish | spanish | reach | |
SPW Buffer | REACH | Portuguese | portuguese | reach | |
SPW Buffer | REACH | Norwegian | norwegian | reach | |
SPW Buffer | REACH | Italian | italian | reach | |
SPW Buffer | REACH | Hungarian | hungarian | reach | |
SPW Buffer | REACH | German | german | reach | |
SPW Buffer | REACH | French | french | reach | |
SPW Buffer | REACH | English | english | reach | |
SPW Buffer | REACH | Dutch | dutch | reach | |
SPW Buffer | REACH | Danish | danish | reach |