Clean-up
Key Applications of Nucleic Acid Clean-up
Clean-up Kits remove inhibitors and other impurities from nucleic acid samples to improve the outcomes of downstream applications like NGS and PCR, among others.
- NGS: Library preparation is the first step of Next-generation sequencing, and DNA cleanup is heavily involved in this step. Achieving inhibitor-free DNA is critical for NGS to reach its full potential
- PCR: Some of the most common sample types, like blood, tissues, and soil, contain many inhibitors. Extracting nucleic acids free of those inhibitors ensures that amplification is successful.
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Implications of Nucleic Acid Purification in Clean-up
- Proper removal of inhibitors like excess nucleotides, primers, and pimer-dimers is imperative for successful library preparation for Next-Generation Sequencing.
- Omega Bio-tek’s products, like Mag-Bind TotalPure NGS and Mag-Bind SeqDTR, are specially designed to eliminate inhibitors and other unwanted components, such as dye terminators, enabling optimized NGS library preparation.
Omega Bio-tek Products for Clean-up Applications
Resources:
Monteiro, D. A., do Rêgo Barros, P. S., Neto, M. D. S. R., da Cruz, H. L. A., Santos, L. R. C., de Arruda Santana, A. C., … & da Costa Rachid, C. T. C. (2022). Use of magnetic beads in Next-Generation Sequencing in the construction of low cost 16S rRNA gene genomic libraries. Pesquisa Agropecuária Pernambucana, 27(2).