E-Z 384 Plant DNA HT Kit

$207.70$714.20

High-throughput isolation of genomic DNA from a variety of plant tissue samples in 384-well format.

Features and Benefits

  • Productivity – Allows processing of up to 768 samples in parallel
  • Robust – Reliable results from a variety of sample types
  • Safe – No organic extractions
  • Performance – High-quality DNA sufficient for most applications

The E-Z 384 Plant DNA HT Kit enables rapid and highthroughput isolation of genomic DNA from a wide variety of plant tissue samples. Up to 768 fresh, frozen, or dried plant tissue samples rich in polyphenols, polysaccharides or having a lower DNA content can be processed in approximately 1.5 hours when processing two plates in parallel. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of silica plate technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. The purification procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with a unique binding system to provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB-based lysis methods. The eluted DNA is inhibitor-free and ready for downstream applications such as genotyping, plant breeding, population level analyses, Next Generation Sequencing, PCR, and more.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting MaterialFresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a low DNA content
Starting Amount30 mg fresh/10 mg dry plant sample per well
Elution Volume65 µL
Processing ModeManual (Centrifugation or Vacuum)
Throughput1 – 768 (process two 384-plates in parallel)
Nucleic Acid Binding Technology384-well silica filter plate
Processing time1.5 h
Downstream Application(s)Genotyping, plant breeding, population level analyses, next generation sequencing, PCR, and more.

Average Yield

Table 1. The average DNA yield from 2 leaf punches of each sample type extracted using the E-Z 384 Plant DNA HT Kit and eluted in 65 μL volume. The DNA yield was determined using Promega’s QuantiFluor® dsDNA system.

Average Ct Values

Figure 1. Average Ct values obtained amplifying the purified DNA from various plant samples using the E-Z 384 Plant DNA HT Kit. The ΔCt values were ~3.3, indicating high-quality DNA that is free of inhibitors.

Extraction Method Comparison

Figure 2. The average DNA yield from 2 leaf punches of canola leaves (n=10) extracted following centrifugation and vacuum protocols of the E-Z 384 Plant DNA HT Kit is shown on the left. The purified DNA is eluted in 65 ul volume and DNA yield was determined using Promega’s QuantiFluor®dsDNA System. The average Ct values were obtained amplifying the purified DNA (right). The ΔCt values were ~3.5, indicating high-quality DNA that is free of inhibitors.

Product Information

NameDocumentLanguagesLink
Protocol ManualProduct ManualEnglish
E-Z 384 Plant DNA HT KitProduct LiteratureEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
CSPL BufferGHSSpanishspanishghs
CSPL BufferGHSEnglishenglishghs
CSPL BufferWHMSFrenchfrenchwhms
CSPL BufferWHMSEnglishenglishwhms
CSPL BufferREACHSwedishswedishreach
CSPL BufferREACHSpanishspanishreach
CSPL BufferREACHNorwegiannorwegianreach
CSPL BufferREACHItalianitalianreach
CSPL BufferREACHGermangermanreach
CSPL BufferREACHFrenchfrenchreach
CSPL BufferREACHFinnishfinnishreach
CSPL BufferREACHDanishdanishreach
CSPL BufferREACHEnglishenglishreach
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferREACHGermangermanreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHDutchdutchreach
Elution BufferWHMSFrenchfrenchwhms
Elution BufferWHMSEnglishenglishwhms
Elution BufferREACHSwedishswedishreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHGermangermanreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHEnglishenglishreach
Elution BufferGHSSpanishspanishghs
Elution BufferGHSEnglishenglishghs
Proteinase K SolutionREACHPortugueseportuguesereach
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHFinnishfinnishreach
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionGHSEnglishenglishghs
Proteinase K SolutionREACHNorwegiannorwegianreach
RNase AREACHSwedishswedishreach
RNase AWHMSFrenchfrenchwhms
RNase AWHMSEnglishenglishwhms
RNase AREACHSpanishspanishreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHItalianitalianreach
RNase AREACHFrenchfrenchreach
RNase AREACHFinnishfinnishreach
RNase AREACHDanishdanishreach
RNase AREACHEnglishenglishreach
RNase AGHSSpanishspanishghs
RNase AGHSEnglishenglishghs
RNase AREACHGermangermanreach
VHB BufferREACHPortugueseportuguesereach
VHB BufferREACHHungarianhungarianreach
VHB BufferREACHDutchdutchreach
VHB BufferWHMSFrenchfrenchwhms
VHB BufferWHMSEnglishenglishwhms
VHB BufferREACHSwedishswedishreach
VHB BufferREACHSpanishspanishreach
VHB BufferREACHNorwegiannorwegianreach
VHB BufferREACHItalianitalianreach
VHB BufferREACHGermangermanreach
VHB BufferREACHFrenchfrenchreach
VHB BufferREACHFinnishfinnishreach
VHB BufferREACHDanishdanishreach
VHB BufferREACHEnglishenglishreach
VHB BufferGHSSpanishspanishghs
VHB BufferGHSEnglishenglishghs
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